Human Astrocyte cDNA

 

Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 110 ng total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40  final volume. RT Reaction stopped by heating at 70°C for 10 minutes. The cDNA is in 1x RT buffer. (1x RT Buffer: 50 mM Tris-Cl, pH 8.3, 75 mM KCI, 3 mM MgCl2, 10mM DTT). The estimated cDNA concentration is about 2.5 . 1 cDNA is good enough for one PCR reaction.

 

Source: Human Brain Tissue

 

Storage Conditions: -20°C or colder