AP2506a-ev | IKB alpha (IKBA) Antibody Clinisciences

All lanes : Anti-Sumo-site. NFkB Antibody at 1:2000 dilution Lane 1: Daudi whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-IKB alpha (IKBA) Antibody at 1:2000 dilution + THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, E
Primary Accession	P25963
Other Accession	NP_065390
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	35609 Da
Antigen Region	1-30 aa

Additional Information
Gene ID	4792
Other Names	NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI
Target/Specificity	This IKB alpha (IKBA) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human IKB alpha (IKBA).
Dilution	WB~~1:2000
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	IKB alpha (IKBA) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	NFKBIA
Synonyms	IKBA, MAD3, NFKBI
Function	Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:1493333, PubMed:36651806, PubMed:7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7479976, PubMed:7628694, PubMed:7796813, PubMed:7878466).
Cellular Location	Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.

IKB alpha (IKBA) Antibody