B5165-5mg | Ionomycin calcium salt [56092-82-1] Clinisciences

Background

Ionomycin calcium salt is a calcium ionophore [1].

Ca2+ ionophore acts as a Ca2+ ionophore, allowing Ca2+ to cross cell membranes.

Ionomycin calcium salt is a calcium ionophore. In cultured chicken pectoralis muscle, ionomycin (10-6-10-5 M) selectively increased the incorporation of methionine into two proteins. Ionomycin increased influx of extracellular Ca2+ and increased Ca2+ concentration in the cytoplasm, which then regulated the synthesis of muscle proteins [2]. In rat parotid gland, ionomycin increased 86Rb efflux, 22Na uptake and 3H-protein secretion, which were dependent on increasing cytosolic Ca2+. In parotid cells, ionomycin released the receptor-regulated cellular Ca2+ pool [3]. In human bladder cancer cells HT1376, ionomycin significantly inhibited cell growth in a time- and dose-dependent way and induced apoptotic DNA degradation. Also, ionomycin reduced the ratios of Bcl-2 to Bax mRNA and protein [4].

In athymic nude mice bearing HT1376 tumors, ionomycin significantly inhibited tumor growth and reduced the tumorigenicity [4].

References:
[1]. Liu C, Hermann TE. Characterization of ionomycin as a calcium ionophore. J Biol Chem, 1978, 253(17): 5892-5894.
[2]. Roufa D, Wu FS, Martonosi AN. The effect of Ca2+ ionophores upon the synthesis of proteins in cultured skeletal muscle. Biochim Biophys Acta, 1981, 674(2): 225-237.
[3]. Poggioli J, Leslie BA, McKinney JS, et al. Actions of ionomycin in rat parotid gland. J Pharmacol Exp Ther, 1982, 221(1): 247-253.
[4]. Miyake H, Hara I, Yamanaka K, et al. Calcium ionophore, ionomycin inhibits growth of human bladder cancer cells both in vitro and in vivo with alteration of Bcl-2 and Bax expression levels. J Urol, 1999, 162(3 Pt 1): 916-921.

Product Citation

1. Dijin Xu, Weiqian Jiang, et al. "PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection." Nature. 2023 Jul;619(7971):819-827. PMID: 37438530

Chemical Properties

Physical Appearance	A crystalline solid
Storage	Desiccate at -20°C
M.Wt	747.08
Cas No.	56092-82-1
Formula	C₄₁H₇₀O₉·Ca
Solubility	Soluble in DMSO
Chemical Name	calcium (4S,6S,8S,10E,12R,14R,16E,18S,19S,20R,21S)-19,21-dihydroxy-22-((2R,2'R,5R,5'S)-5'-((R)-1-hydroxyethyl)-2,5'-dimethyloctahydro-[2,2'-bifuran]-5-yl)-4,6,8,12,14,18,20-heptamethyl-11-oxido-9-oxodocosa-10,16-dienoate
SDF	Download SDF
Canonical SMILES	O[C@H](C)[C@@]1(C)O[C@H](CC1)[C@]2(C)O[C@@H](C[C@@H]([C@@H](C)[C@H]([C@H](/C=C/C[C@@H](C)C[C@@H](C)/C([O-])=C\C([C@@H](C)C[C@@H](C)C[C@@H](C)CCC([O-])=O)=O)C)O)O)CC2.[Ca+2]
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment:[1]
Cell lines	Human bladder cancer cell line HT1376
Reaction Conditions	0 ~ 100 μg/ml ionomycin for 0 ~ 96 h incubation
Applications	The in vitro growth rate of HT1376 cells was suppressed by ionomycin in a dose- and time-dependent manner. After continuous treatment with 10 μg/ml ionomycin, characteristic apoptotic DNA degradation, as well as marked decreases in the ratios of Bcl-2 to Bax mRNA and protein could be observed in HT1376 cells.
Animal experiment:[1]
Animal models	Athymic nude mice bearing HT1376 tumors
Dosage form	50 μg of ionomycin in 100 μl PBS Injected intratumorally three times weekly for 4 weeks
Applications	Intratumoral injection of ionomycin into subcutaneous HT1376 tumors reduced the tumorigenicity in nude mice. Furthermore, these in vivo growth-inhibitory effects of ionomycin were significantly enhanced by pretreatment with cisplatin.
Note	The technical data provided above is for reference only.
	References: 1. Miyake H, Hara I, Yamanaka K, et al. Calcium ionophore, ionomycin inhibits growth of human bladder cancer cells both in vitro and in vivo with alteration of Bcl-2 and Bax expression levels. The Journal of Urology, 1999, 162(3 Pt 1): 916-921.

Quality Control

Purity = 98.00%

Ionomycin calcium salt [56092-82-1]

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