LGI4 (NM_139284) Human Untagged Clone

Referentie SC313909

Formaat : 10ug

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LGI4 (NM_139284) Human Untagged Clone

Specifications
Product Data
Target Symbol LGI4
Synonyms AMC1; AMCNMY; LGIL3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC313909 representing NM_139284.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGGAGGGGCAGGCATTCTGCTGCTGCTGCTGGCTGGGGCGGGGGTGGTGGTGGCCTGGAGACCCCCA
AAGGGAAAGTGTCCCCTGCGCTGCTCCTGCTCTAAAGACAGCGCCCTGTGTGAGGGCTCCCCGGACCTG
CCCGTCAGCTTCTCTCCGACCCTGCTGTCACTCTCACTCGTCAGGACGGGAGTCACCCAGCTGAAGGCC
GGCAGCTTCCTGAGAATTCCGTCTCTGCACCTGCTCCTCTTCACCTCCAACTCCTTCTCCGTGATTGAG
GACGATGCATTTGCGGGCCTGTCCCACCTGCAGTACCTCTTCATCGAGGACAATGAGATTGGCTCCATC
TCTAAGAATGCCCTCAGAGGACTTCGCTCGCTTACACACCTAAGCCTGGCCAATAACCATCTGGAGACC
CTCCCCAGATTCCTGTTCCGAGGCCTGGACACCCTTACTCATGTGGACCTCCGCGGGAACCCGTTCCAG
TGTGACTGCCGCGTCCTCTGGCTCCTGCAGTGGATGCCCACCGTGAATGCCAGCGTGGGGACCGGCGCC
TGTGCGGGCCCCGCCTCCCTGAGCCACATGCAGCTCCACCACCTCGACCCCAAGACTTTCAAGTGCAGA
GCCATAGAGCTGTCCTGGTTCCAGACGGTGGGGGAGTCGGCACTGAGCGTAGAGCCCTTCTCCTACCAA
GGGGAGCCTCACATTGTGCTGGCACAGCCCTTCGCCGGCCGCTGCCTGATTCTCTCCTGGGACTACAGC
CTGCAGCGCTTCCGGCCCGAGGAAGAGCTGCCCGCGGCCTCCGTGGTGTCCTGCAAGCCACTGGTGCTG
GGCCCGAGCCTCTTCGTGCTGGCTGCCCGCCTGTGGGGGGGCTCACAGCTGTGGGCCCGGCCCAGTCCC
GGCCTGCGCCTGGCCCCAACGCAGACCCTGGCCCCGCGGCGGCTGCTGCGGCCCAATGACGCCGAGCTC
CTGTGGCTGGAAGGGCAACCCTGCTTCGTGGTGGCCGATGCCTCCAAGGCGGGCAGCACCACGCTGCTG
TGCCGCGACGGGCCCGGCTTTTACCCGCACCAGAGCCTGCACGCCTGGCACCGGGACACGGACGCTGAG
GCCCTGGAGCTGGACGGCCGGCCCCACCTGCTGCTGGCCTCGGCTTCCCAGCGGCCCGTGCTCTTCCAC
TGGACCGGTGGCCGCTTCGAGAGACGCACAGACATCCCCGAGGCCGAGGATGTCTATGCCACACGCCAC
TTCCAGGCTGGTGGGGACGTGTTCCTGTGCCTCACACGCTACATTGGGGACTCCATGGTCATGCGCTGG
GACGGCTCCATGTTTCGTCTGCTGCAGCAACTTCCCTCGCGCGGTGCCCACGTCTTCCAGCCACTGCTC
ATCGCCAGGGACCAGCTGGCCATCCTAGGCAGCGACTTCGCCTTCAGCCAGGTCCTCCGCCTTGAGCCT
GACAAGGGGCTCCTGGAGCCACTGCAGGAGCTGGGGCCTCCGGCCCTGGTGGCCCCCCGTGCCTTTGCC
CACATCACTATGGCCGGCAGACGCTTCCTCTTTGCTGCTTGCTTTAAGGGCCCCACACAGATCTACCAG
CATCACGAGATCGACCTCAGTGCCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI Plasmid Map
ACCN NM_139284
Insert Size 1614 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_139284.2
RefSeq Size 2906 bp
RefSeq ORF 1614 bp
Locus ID 163175
UniProt ID Q8N135
Cytogenetics 19q13.11
Domains EPTP, LRR, LRRCT, LRR_TYP
MW 59.1 kDa
Summary Component of Schwann cell signaling pathway(s) that controls axon segregation and myelin formation (By similarity).[UniProtKB/Swiss-Prot Function]
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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