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Mouse Monoclonal Anti-PAX6 (Stem Cell Marker) [Clone SPM612]
Referentie NB-36-00586X-P1
Formaat : 100ug
Merk : Neo Biotech
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Printable Datasheet
PAX6 (Stem Cell Marker) Antibody [SPM612]
Catalog Number Formulation Size Price
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with PAX6 Monoclonal Antibody (SPM612).
Applications & Dilutions
Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Summary
Pax genes contain paired domains with strong homology to genes in Drosophila, which are involved in programming early development. Lesions in the Pax-6 gene account for most cases of aniridia, a congenital malformation of the eye, chiefly characterized by iris hypoplasia, which can cause blindness. Pax-6 is involved in other anterior segment malformations besides aniridia, such as Peters' anomaly, a major error in the embryonic development of the eye with corneal clouding with variable iridolenticulocorneal adhesions. The Pax-6 gene encodes a transcriptional regulator that recognizes target genes through its paired-type DNA-binding domain. The paired domain is composed of two distinct DNA-binding subdomains, the amino-terminal subdomain and the carboxy-terminal subdomain, which bind respective consensus DNA sequences. The human Pax-6 gene produces two alternatively spliced isoforms that have the distinct structure of the paired domain.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Autophagy, Developmental Biology, Neural Stem Cells, Nuclear Marker, Stem Cell Differentiation
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus
Gene Name
PAX6
Positive Control
Cerebellum or Stomach., Pancreas
Immunogen
Recombinant fragment (N-terminus; aa 1-300) of human PAX6 protein
Alternate Names
Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, AN2; Aniridia type II protein; Oculorhombin; Paired box gene 6 (aniridia keratitis); Paired box homeotic gene 6; Paired box protein Pax-6; Sey; WAGR
Functions
- Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Acts as a transcriptional repressor of NFATC1-mediated gene expression (By similarity).
Key References
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/1684738/
- https://pubmed.ncbi.nlm.nih.gov/1345175/
- https://pubmed.ncbi.nlm.nih.gov/17974005/
- https://pubmed.ncbi.nlm.nih.gov/16554811/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/7958875/
- https://pubmed.ncbi.nlm.nih.gov/7668281/
- https://pubmed.ncbi.nlm.nih.gov/18669648/
- https://pubmed.ncbi.nlm.nih.gov/19414065/
- https://pubmed.ncbi.nlm.nih.gov/20068231/
- https://pubmed.ncbi.nlm.nih.gov/24290376/
- https://pubmed.ncbi.nlm.nih.gov/10346815/
- https://pubmed.ncbi.nlm.nih.gov/9482572/
- https://pubmed.ncbi.nlm.nih.gov/8364574/
- https://pubmed.ncbi.nlm.nih.gov/8162071/
- https://pubmed.ncbi.nlm.nih.gov/8640214/
- https://pubmed.ncbi.nlm.nih.gov/9147640/
- https://pubmed.ncbi.nlm.nih.gov/9281415/
- https://pubmed.ncbi.nlm.nih.gov/9792406/
- https://pubmed.ncbi.nlm.nih.gov/9538891/
- https://pubmed.ncbi.nlm.nih.gov/9856761/
- https://pubmed.ncbi.nlm.nih.gov/10441571/
- https://pubmed.ncbi.nlm.nih.gov/10234503/
- https://pubmed.ncbi.nlm.nih.gov/9931324/
- https://pubmed.ncbi.nlm.nih.gov/10955655/
- https://pubmed.ncbi.nlm.nih.gov/10737978/
- https://pubmed.ncbi.nlm.nih.gov/11553050/
- https://pubmed.ncbi.nlm.nih.gov/11309364/
- https://pubmed.ncbi.nlm.nih.gov/11826019/
- https://pubmed.ncbi.nlm.nih.gov/12721955/
- https://pubmed.ncbi.nlm.nih.gov/12634864/
- https://pubmed.ncbi.nlm.nih.gov/12552561/
- https://pubmed.ncbi.nlm.nih.gov/16493447/
- https://pubmed.ncbi.nlm.nih.gov/17595013/
- https://pubmed.ncbi.nlm.nih.gov/21850189/
- https://pubmed.ncbi.nlm.nih.gov/24033328/
- https://pubmed.ncbi.nlm.nih.gov/29914532/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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