mRNA Magnetic Purification Kit
Referentie K1307-5ml
Formaat : 5ml
Merk : APExBIO Technology
Neem contact op met een lokale distributeur :
Telefoonnummer : +1 850 650 7790
mRNA Magnetic Purification Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
mRNA Magnetic Purification Kit is designed for purifying mRNA from total RNA or in vitro transcription, which uses monodisperse superparamagnetic beads that covalently couple oligomeric dT sequences on its surface. High purity mRNA is achieved by base pairing between the oligo-DT sequence and the poly(A) tail of the mRNA. The dT sequence combined with mRNA can be directly used as the first chain reaction primer to synthesize cDNA, or mRNA can be eluted from the beads for various downstream molecular experiments, such as RT-PCR, RPA (ribonuclease protection assay), library construction, Northern Blot analysis, next-generation sequencing, etc.
| Components | 2 mL | 5 mL |
| Oligo (dT) 25 beads (10 mg/mL) | 2*1 mL | 5*1 mL |
| Binding Buffer | 5 mL | 3*5 mL |
| Washing Buffer | 5 mL | 3*5 mL |
| Tris-HCl Buffer | 1 mL | 3*1 mL |
| RNase-free water | 2*1 mL | 5*1 mL |
Store at 4 °C, no freezing! | ||