Myogenin (APC)

Referentie 138574-AF-APC-100ul

Formaat : 100ul

Merk : US Biological

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138574-AF-APC Myogenin (APC)

Clone Type
Polyclonal
Host
mouse
Source
human
Swiss Prot
P15173, P12979, P49812
Isotype
IgG1,k
Grade
Affinity Purified
Applications
FLISA IF IHC IP WB
Crossreactivity
Fe Hu Mo Po Rt
Accession #
NP_002470
Gene #
MYOG
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70aa in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% aa homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms' tumor.||Applications:|Suitable for use in FLISA, Western Blot, Flow Cytometry (Not Tested), Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Flow Cytometry (Not Tested): 0.5-1ug/10e6 cells|Immunofluorescence: 1-2ug/ml|Western Blot: 0.5-1ug/ml|Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT|Immunoprecipitation: 1-2ug/500ug protein lysate|Optimal dilutions to be determined by the researcher.||Positive Control:|Rh-30 cells. Skeletal muscle or rhabdomyosarcoma||Storage and Stability:|May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1,k|Clone No: MGN185|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein G affinity chromatography.|Immunogen: Recombinant protein corresponding to human myogenin.|Specificity: Recognizes human myogenin. Species Crossreactivity: mouse, rat, feline and porcine. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Recombinant protein corresponding to human myogenin.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human myogenin. Species Crossreactivity: mouse, rat, feline and porcine.
References
1. Wang NP et. al. Am J Pathol 1995, 147:1799-1810.