Narcissus pseudonarcissus Lectin (NPA, NPL)

Referentie N0204-10-5mg

Formaat : 5mg

Merk : US Biological

Meer informatie aanvragen

Neem contact op met een lokale distributeur :


Telefoonnummer : +1 850 650 7790


N0204-10 Narcissus pseudonarcissus Lectin (NPA, NPL)

Clone Type
Polyclonal
Grade
Molecular Biology Grade
Shipping Temp
Blue Ice
Storage Temp
-20°C

Daffodil lectin exists as a dimeric protein composed of two subunits of 13,000D devoid of attached carbohydrate. Below pH 5.0 and above pH 9.0 NPL dissociates into monomers. This lectin has a specificity toward alpha linked mannose, preferring polymannose structures containing (a-1,6) linkages. Binding to mannose polymers can occur via internal mannose residues and is not dependent on structural integrity of a non-reducing end sugar. NPL also binds some galactomannans, and differs in other binding characteristics from a related lectin, Galanthus nivalis lectin. Unlike Con A, LCA and PSA, NPL does not bind glucose. Staining of the cytoplasmic membrane of cells with NPL after short-term acid treatment can indicate those cells undergoing apoptosis. Detection of apoptotic cells using NPL binding is a highly sensitive and reliable tool for studying cell death.||Applications: |User-determined||Inhibiting Sugar: |100mM mannose||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Source: Daffodil bulbs|Purity: Homogeneous by SDS-PAGE|Concentration: Not determined |Form: Supplied as a lyophilized powder.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a lyophilized powder.
Purity
Homogeneous by SDS-PAGE