Peanut Lectin (FITC)
Referentie GTX01508
Formaat : 1mg
Merk : GeneTex
Neem contact op met een lokale distributeur :
Telefoonnummer : +1 850 650 7790
Application | ICC/IF |
|---|---|
Species | Arachis hypogaea |
| Package | 1 mg |
Datasheet File View product citations for antibody GTX01508 on CiteAb
PRODUCT
Summary
Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| ICC/IF | 1:250-1:1000 |
Not tested in other applications.
Product Note
Carbohydrate-Binding Specificity of Peanut Lectin : Galβ1, 3 GalNAc>>GalNAc>Gal
PROPERTIES
Form
Liquid
Buffer
10mM Bicarbonate, 150mM NaCl, 0.1mM CaCl₂
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
5 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
Native Protein
Expression System
Native Protein
Purification
Purified by affinity chromatography followed by gel filtration chromatography
Purified from Peanut
Purified from Peanut
Conjugation
Fluorescein isothiocyanate (FITC) Wavelength
Absorption : 490 nm ; Emission : 520 nm. Ratio : 1.0-1.4 molecules FITC (isomer 1) per Lectin molecule.
Absorption : 490 nm ; Emission : 520 nm. Ratio : 1.0-1.4 molecules FITC (isomer 1) per Lectin molecule.
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
PNA lectin , PNA , Arachis hypogaea , Peanut Lectin , Peanut glutinin ctin , Arachis hypogaea , Peanut agglutinin , Arachis hypogaea lectin , Peanut agglutinin lectin
Background
Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells. Peanut lectin contains one atom of Ca++ and Mg++ per unit.
Inhibiting/Eluting sugars: 200 mM GalactoseCarbohydrate-Binding Specificity of PNA: Galβ1, 3 GalNAc>>>>>GalNAc>Gal
REVIEW
| SDS | |
|---|---|
| Sodium Azide.pdf | |
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