B7758-10mg | Propidium iodide [25535-16-4] Clinisciences

Background

Propidium iodide, a cell-membrane impermeable dye, is frequently used to stain DNA, with an excitation maximum at 535 nm and an emission maximum at 617 nm. Although propidium iodide is generally excluded from viable cells with intact membranes, it can easily penetrate dead or damaged cells, which makes it commonly used for identifying cell viability in a population or as a counterstain in multicolor fluorescent techniques. Propidium iodide intercalates with nucleic acids with a stoichiometry of one dye per 4 ~ 5 base pairs with little sequence preference, and is then detected by fluorescence microscopy and flow cytometry. Propidium iodide has been shown to be a useful tool to assess apoptosis of certain cell populations in heterogeneous tissues including bone marrow, lymph nodes and thymus.

References:

1. Coder DM. Assessment of cell viability. Current Protocols in Cytometry, 2001, Chapter 9: Unit 9.2.

2. Hezel M, Ebrahimi F, Koch M, et al. Propidium iodide staining: a new application in fluorescence microscopy for analysis of cytoarchitecture in adult and developing rodent brain. Micron, 2012, 43(10): 1031-1038.

3. Nicoletti I, Migliorati G, Pagliacci MC, et al. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. Journal of Immunological Methods, 1991, 139(2): 271-279.

Product Citation

1. Gianpiero Di Leva, et al. "Tivantinib induces mitotic death in SHH medulloblastoma cell lines." Research Square. 05 Mar, 2024.
2. Yizuo Song, Xin Shou, et al. "Cell Membranes from Tumor‐Tropic MSCs Screened by a Microfluidic Chip for Drug Nanoparticles Encapsulation and Cancer Targeted Therapy." Adv Healthc Mater. 2023 Jul;12(17):e2202904. PMID: 36815538
3. Yang Fu, Runqing Huang, et al. "LncRNA ENSG00000254615 Modulates Proliferation and 5-FU Resistance by Regulating p21 and Cyclin D1 in Colorectal Cancer." Cancer Invest. 2021 Oct;39(9):696-710. PMID: 33938344

Chemical Properties

Physical Appearance	A crystalline solid
Storage	Store at -20°C
M.Wt	668.39
Cas No.	25535-16-4
Formula	C₂₇H₃₄I₂N₄
Solubility	insoluble in H2O; insoluble in EtOH; ≥9.84 mg/mL in DMSO
Chemical Name	3,8-diamino-5-(3-(diethyl(methyl)ammonio)propyl)-6-phenylphenanthridin-5-ium iodide
SDF	Download SDF
Canonical SMILES	NC1=CC2=[N+](C(C3=CC=CC=C3)=C(C=C4N)C(C=C4)=C2C=C1)CCC[N+](CC)(C)CC.[I-].[I-]
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Quality Control

Purity = 99.60%

Propidium iodide [25535-16-4]

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Background

Product Citation

Chemical Properties

Quality Control

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