Protein Phosphatase 2A, Serine, Threonine (PP2A Ser/Thr) (FITC)

Referentie P9107-33-FITC-100ul

Formaat : 100ul

Merk : US Biological

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P9107-33-FITC Protein Phosphatase 2A, Serine, Threonine (PP2A Ser/Thr) (FITC)

Clone Type
Polyclonal
Host
mouse
Source
human
Swiss Prot
P67775
Isotype
IgG2b,k
Grade
Affinity Purified
Applications
IC IP WB
Crossreactivity
Bo Hu Mo Rb Rt Xe Ye
Accession #
NM_004156
Shipping Temp
Blue Ice
Storage Temp
-20°C

Type 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the alpha subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal downmodulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine.||Applications: |Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.||Recommended Dilutions:|Western Blot: 0.5-2ug/ml. Detects PP2A (36kD) in human A431 and in murine 3T3 RIPA cell lysates. |Immunoprecipitation: 4ug immunoprecipitated PP2A from 500ug of A431 RIPA lysate.|Immunocytochemistry: 5-10ug/ml shows positive immunostaining for PP2A, C subunit in A431 fixed for 10 minutes with 4% paraformaldehyde in PBS.|Optimal dilutions to be determined by the researcher.||Recommended Secondary Antibodies:|I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo|I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo|I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo||Positive Antigen Control (Included):|A0001-50: Non stimulated A431 lysate. Load 20ug of reduced lysate per lane for minigels.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2b,k|Clone No: 0.T.118 (1D6)|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified by Protein G affinity chromatography from ascites.|Immunogen: Synthetic peptide corresponding to aa295-309, CRGEPHVTRRTPDYFL of the 36kD catalytic subunit of human Protein Phosphatase 2A (NM_004156).|Specificity: Recognizes human Protein Phosphatase 2A, Serine, Threonine, catalytic subunit, Mr 36kD. Species Crossreactivity: bovine, mouse, rat, rabbit, Xenopus and yeast. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679.|||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic peptide corresponding to aa295-309, CRGEPHVTRRTPDYFL of the 36kD catalytic subunit of human Protein Phosphatase 2A (NM_004156).
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes human Protein Phosphatase 2A, Serine, Threonine, catalytic subunit, Mr 36kD. Species Crossreactivity: bovine, mouse, rat, rabbit, Xenopus and yeast. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679.|
References
1. Kremmer, E., et al., Mol. Cell Bio. 17: 1692-1701, 1997. 2. Kawabe, T., et al., Nature 385: 454-458, 1997. 3. Papasozomenos, S.C., and Y. Su, J. Neurochem. 65: 396-406, 1995. 4. Shima, H., et al., Proc. Natl. Acad. Sci. USA 91: 9267-9271. 5. McMahon, K., et al., J. Biochem. 358: 431-436, 2001. 6. Yu, X., Mol. Biol. Cell 12: 185-199, 2001. 7. Thompson, L., et al., J. Biol. Chem. 272: 29693-296697, 1997.