OKCD02284 | Quinolinic Acid ELISA Kit Clinisciences

Datasheets/Manuals	Printable datasheet for Quinolinic Acid ELISA Kit (OKCD02284)
COA Datasheet	Click Here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.

Product Info

Predicted Species Reactivity

All Species

Application

ELISA-Comp

ELISA Kit Detection Method

Colorimetric

ELISA Kit Duration

2h

ELISA Kit Linearity

Matrix	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
serum(n=5)	82-101%	80-99%	96-105%	89-96%
EDTA plasma(n=5)	93-101%	84-96%	99-105%	83-104%
heparin plasma(n=5)	81-99%	91-102%	94-105%	98-105%

ELISA Kit Principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Quinolinic Acid (QA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Quinolinic Acid (QA) and unlabeled Quinolinic Acid (QA) (Standards or samples) with the pre-coated antibody specific to Quinolinic Acid (QA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Quinolinic Acid (QA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Quinolinic Acid (QA) in the sample.

ELISA Kit Range

1.23-100ng/mL

ELISA Kit Recovery

Matrix	Recovery Range (%)	Average (%)
serum(n=5)	90-98	94
EDTA plasma(n=5)	81-99	93
heparin plasma(n=5)	90-98	93

ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Quinolinic Acid (QA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Quinolinic Acid (QA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

ELISA Kit Component

Component	Amount
Anti-Quinolinic Acid Microplate	96 Wells (12 x 8 Well Strips)
Quinolinic Acid Lyophilized Standard	2
100X Quinolinic Acid Biotin Complex	120 uL
100X Avidin/HRP Conjugate	120 uL
Standard Diluent	1 x 20 mL
Biotin Complex Diluent	1 x 12 mL
Avidin/HRP Conjugate Diluent	1 x 12 mL
30X Wash Buffer	1 x 20 mL
TMB Substrate	1 x 9 mL
Stop Solution	1 x 6 mL

Additional Information

CAS: 89-00-9
PubChem: 1066

Reconstitution and Storage

2°C to 8°C|-20°C

Sample Type

Serum, plasma and other biological fluids

Sensitivity

0.58 ng/mL

Publications

Abdur Rahman, Muddanna S. Rao & Khalid M. Khan. Intraventricular infusion of quinolinic acid impairs spatial learning and memory in young rats: a novel mechanism of lead-induced neurotoxicity. J Neuroinflammation. 15, 263 (2018). PMC613774330980800

Assay Info

Assay Methodology: Competitive Inhibition Immunoassay

Gene Symbol	QA
Alias Symbols	Quinolinic acid; 2,3-pyridinedicarboxylic acid; Pyridine-2,3-dicarboxylic acid; 89-00-9; Quinolinate; Pyridine-2,3-dicarboxylate
Protein Name	Quinolinic Acid
Description of Target	Quinolinic acid is a metabolite of tryptophan with a possible role in neurodegenerative disorders. Elevated CSF levels of quinolinic acid are correlated with the severity of neuropsychological deficits in patients who have AIDS.

Quinolinic Acid ELISA Kit

Referentie OKCD02284

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