RABBIT IgG (H&L) antibody (TRITC)
Referentie orb347722-1mg
Formaat : 1mg
Merk : Biorbyt
RABBIT IgG (H&L) antibody (TRITC)
Catalog Number: orb347722
Dot Blot results of Mouse Anti-Rabbit IgG Antibody Rhodamine Conjugated. Dots are Rabbit IgG at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 60 min at RT. Primary Antibody: none. Secondary Antibody: Mouse Anti-Rabbit IgG TRITC at 1 µg/mL for 1 hr at RT.
Western Blot of Anti-Rabbit IgG (H&L) (MOUSE) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n orb347723). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment (p/n orb346280). Lane 4: Rabbit IgM Whole Molecule (p/n orb346313). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (MOUSE) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n orb347723) 1:1000 for 60 min at RT. Secondary antibody: Anti-Mouse IgG (RABBIT) Peroxidase Conjugated Antibody (p/n orb347506) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.