Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 25-110aa
- UniProt No.
- P01308
- NCBI Accession No.
- NP_000198
- Alternative names
- Insulin preproprotein, IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10
Product Specification
- Molecular Weight
- 11.8 kDa (109aa) confirmed by MALDI-TOF
- Concentration
- 0.5mg/ml (determined by absorbance at 280nm)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Measured in a cell proliferation assay using MCF7 human breast cancer cell. The ED50 range ≤ 4ug/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Human Proinsulin in a cell proliferation assay using MCF7 human breast cancer cell. The ED50 range ≤ 4ug/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.