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> SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit

SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit

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Referentie GTX536267

Formaat : 96test

Merk : GeneTex

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SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit. GTX536267
SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit. GTX536267
SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit. GTX536267
SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit. GTX536267
SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit. GTX536267
SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit. GTX536267
SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit. GTX536267
SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit. GTX536267

Application

 ELISA, Sandwich ELISA

Reactivity

 SARS Coronavirus 2
Package
96 test
Datasheet File
View product citations for antibody GTX536267 on CiteAb
PRODUCT

Summary

The GeneTex SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA kit is an in vitro tool that quantitatively detects the SARS-CoV-2 Spike receptor-binding domains (RBDs) of the "wildtype" SARS-CoV-2 strain as well as those of the Alpha, Beta, Gamma, Delta, Alpha (E484K), and Kappa variants. This assay employs a pair of monoclonal antibodies specific for the SARS-CoV-2 RBD, with the lowest detectable dose concentration of standard RBD protein being 1.8 pg/ml (Alpha variant).

APPLICATION

Application Note

The suitable sample types include cell culture supernatant, plasma and serum. The suggested sample loading amount is 50μl per well. Please see the protocol for detailed procedure. Assay range : Please refer to the protocol.

Positive Control

S1 Standard (lyophilized recombinant S1 protein)

Product Note

This ELISA kit detects Spike RBD proteins derived from multiple SARS-CoV-2 VOCs, including Omicron variant (BA.1 and BA.2).

PROPERTIES

Storage

The entire kit can be stored at 2-8ºC for up to 6 months.

Conjugation

Horseradish peroxidase(HRP)

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit. GTX536267

GTX536267 ELISA Image

Sandwich ELISA detection of recombinant Spike ECD proteins derived from different strains of SARS-CoV-2 virus (ie., wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit (GTX536267).

SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit. GTX536267

GTX536267 ELISA Image

Sandwich ELISA detection of recombinant Spike RBD proteins derived from different strains of SARS-CoV-2 (ie., wild type; B.1.1.7 Alpha variant; B.1.351 Beta variant; P.1 Gamma variant; B1.617.2 Delta variant, B.1.617.1 Kappa variant; BA.1 Omicron variant; BA.2 Omicron variant; B.1.1.7 Alpha variant with E484K) using SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit (GTX536267).

SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit. GTX536267

GTX536267 ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein (GTX135972-pro) derived from wild-type strain of SARS-CoV-2 using SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit (GTX536267).

SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit. GTX536267

GTX536267 ELISA Image

Sandwich ELISA detection of recombinant Spike RBD proteins derived from different strains of SARS-CoV-2 (ie., wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4 / BA.5 Omicron variant; BF.7 Omicron variant; BQ.1 Omicron variant; XBB Omicron variant) using SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit (GTX536267).

SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit. GTX536267

GTX536267 ELISA Image

ELISA analysis of SARS-CoV-2 (COVID-19) Spike protein S1 subunit using SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit (GTX536267).

SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit. GTX536267

GTX536267 ELISA Image

ELISA assay using lysates of 293T cells overexpressing the spike proteins from SARS-CoV-2, SARS-CoV, or MERS-CoV. The SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit (GTX536267) specifically detects only SARS-CoV-2 spike protein.

SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit. GTX536267

GTX536267 ELISA Image

A. Recovery assay was determined by spiking SARS-CoV-2 spike and RBD proteins at various levels throughout the assay range in the listed samples. B. To determine linearity, the listed samples were spiked with SARS-CoV-2 spike and RBD proteins and diluted with Assay Diluent in 1:2 and 1:4. The value was assessed by comparing it to undiluted samples.

SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit. GTX536267

GTX536267 Image

Application Reference

A Altay Benetti et al. Vaccines (Basel) 2024; 12 (4) : 409 Design and Characterization of a New Formulation for the Delivery of COVID-19-mRNA Vaccine to the Nasal Mucosa
Bae KH et al. NPJ Vaccines 2024; 9 (1) : 43 Durable cross-protective neutralizing antibody responses elicited by lipid nanoparticle-formulated SARS-CoV-2 mRNA vaccines.
Kim K et al. Front Immunol 2022; 13 : 1020165 Translation suppression underlies the restrained COVID-19 mRNA vaccine response in the high-risk immunocompromised group.

REVIEW

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Application Reference
A Altay Benetti et al. Vaccines (Basel) 2024; 12 (4):409 Design and Characterization of a New Formulation for the Delivery of COVID-19-mRNA Vaccine to the Nasal Mucosa
Bae KH et al. NPJ Vaccines 2024; 9 (1):43 Durable cross-protective neutralizing antibody responses elicited by lipid nanoparticle-formulated SARS-CoV-2 mRNA vaccines.
Kim K et al. Front Immunol 2022; 13:1020165 Translation suppression underlies the restrained COVID-19 mRNA vaccine response in the high-risk immunocompromised group.
SDS
SDS-GTX ELISA kitv2.2.pdf

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