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> SARS-CoV-2 Nucleocapsid Protein IgG ELISA Kit

SARS-CoV-2 Nucleocapsid Protein IgG ELISA Kit

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Referentie E-EL-E600-10x96T

Formaat : 10x96T

Merk : Elabscience

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Product Summary
Sample Volume 100 μL
Total Assay Time 1 h 30 min
Reacitivity Human
Specificity This kit recognizes Human SARS-CoV-2 IgG in samples.No significant cross-reactivity or interference between Human SARS-CoV-2 IgG and analogues was observed
Recovery 80%-120%
Sample Type serum, plasma
Detection Method Colorimetric method, ELISA, Indirect
Assay Type Indirect-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 6 months
This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the SARS-CoV-2 Nucleocapsid protein IgG in the sample. The micro ELISA plate provided in this kit is pre-coated with purified SARS-CoV-2 Nucleocapsid protein (E-ELC-001) antigen, after adding samples to wells, the SARS-CoV-2 Nucleocapsid protein IgG in the samples will combine with the pre-coated SARS-CoV-2 Nucleocapsid protein antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated mouse anti human IgG to develop the antigen-antibody-HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Nucleocapsid protein IgG and HRP conjugated anti-human IgG will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Nucleocapsid protein IgG exists in the tested samples or not.
SARS-CoV-2 N protein IgG antibody is an IgG type of antibody produced against the N protein (nucleocapsid protein) of the novel coronavirus (SARS-CoV-2). N protein is an important structural protein of SARS-CoV-2 virus, which plays a key role in the process of virus replication and assembly
Research Area SARS-CoV-2 , COVID 19
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