SARS, Spike Protein, Mosaic , Recombinant

The spike (S) glycoprotein of coronaviruses mediates viral entry into host cells. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. It is a type 1 viral fusion protein that characteristically contains two heptad repeat regions, denoted HR-N and HR-C, that form coiled-coil structures within the ectodomain of the protein. Previous studies have shown that the two heptad repeat regions can undergo a conformational change from their native state to a 6-helix bundle (trimer of dimers), which mediates fusion of viral and host cell membranes||SARS-ACSM contains the middle section of the Spike protein immunodominant regions, amino acid fragments: (12-53), (90-115), (171-203).||Specificity:|Immunoreactive with sera of SARS-infected individuals||Application:|Recombinant SARS-ACSM Antigen may be used in ELISA and Western Blot, excellent for detection of SARS with minimal specificity problems.||Storage and Stability:|May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.