K2606-20ml | Tartrate Resistant Acid Phosphatase (TRAP) staining Kit

Description

The acid phosphatase (ACP) family is a class of enzymes that are widely found in various tissues, mainly in the lysosomes of cells, so they are often used as markers of lysosomes. Tartrate-resistant acid phosphatase (TRAP) is the most dominant enzyme in the ACP family, found in alveolar macrophages in normal humans and splenocytes in patients with leukemia, and is usually located in the follicles of cells and is not released into the blood. TRAP in the blood is mainly derived from osteoclasts, therefore, by measuring the level of TRAP in the blood, the activity of osteoclasts can be assessed.

The Tartrate Resistant Acid Phosphatase (TRAP) staining kit uses naphthol AS-BI as a substrate, which can be catalyzed by TRAP in an acidic environment to produce phosphoric acid and naphthol. Subsequently, naphthol reacts with diazonium salts to form colored conjugates and is detected. This kit can be used for fresh blood smears, cell smears, and can also be used for the detection of frozen sections and paraffin sections.

Figure 1: Validation diagram of this kit with hematoxylin counterstain(left) or methyl green counterstain (right)

Quality Control

Protocol

Components and Storage

Components	K2606-10 mL	K2606-20 mL
GBC Solution A	100 μL	200 μL
GBC Solution B	100 μL	200 μL
AS-BI Staining Solution	100 μL	200 μL
TRAP Buffer	9.7 mL	19.4 mL
Hematoxylin Staining Solution	10 mL	20 mL
Methyl Green Staining Solution	10 mL	20 mL
Store TRAP Buffer and Methyl Green Staining Solution at room temperature away from light. Store Hematoxylin Staining Solution at 4°C away from light. Store other reagents at -20°C away from light. This kit is stable for 3 months.

Tartrate Resistant Acid Phosphatase (TRAP) staining Kit

Referentie K2606-20ml

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Description

Quality Control

Components and Storage

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