Biological Activity

3,3’,5,5’-Tetramethylbenzidine(TMB) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).

Chemical Information

Molecular Weight	240.34
Formula	C16H20N2
CAS Number	54827-17-7
Solubility (25°C)	DMSO: ≥ 10 mg/mL
Storage	2-8°C, dry, protect from light, sealed

References

[1] Sara Bravaccini, et al. TMB in NSCLC: A Broken Dream?

[2] Alfredo Addeo, et al. TMB or not TMB as a biomarker: That is the question

[3] Alessandro Rizzo, et al. PD-L1, TMB, MSI, and Other Predictors of Response to Immune Checkpoint Inhibitors in Biliary Tract Cancer

[4] Diana M Merino, et al. Establishing guidelines to harmonize tumor mutational burden (TMB): in silico assessment of variation in TMB quantification across diagnostic platforms: phase I of the Friends of Cancer Research TMB Harmonization Project

[5] Li Liu, et al. Combination of TMB and CNA Stratifies Prognostic and Predictive Responses to Immunotherapy Across Metastatic Cancer