TransDetect® Cell LIVE/DEAD Viability/Cytotoxicity Detection Kit
Referentie FC301-01
Formaat : 100rxns
Merk : TransGen Biotech
The TransDetect® Cell LIVE/DEAD Viability/Cytotoxicity Detection Kit provides a simple, high-specificity and low-toxicity way to distinguish between live and dead cells by combining Calcein-AM and Propidium iodide (PI). Quantification or sorting can be performed by flow cytometry.
Calcein-AM is a low-cytotoxicity non-fluorescent dye, which easily permeates intact live cells. In live cells, the Calcein-AM is converted to green fluorescent calcein with excitation and emission wavelengths of 490/515 nm by intracellular esterase digestion. PI is excluded by intact plasma membrane of live cells. It is a DNA-binding fluorescent dye with excitation and emission wavelengths of 535/617 nm, producing a bright red fluorescence in dead cells with broken membrane.
• Higher specificity.
• More simplicity.
• Lower cytotoxicity.
Storage
at -20℃ in the dark for up to one year.
Shipping
Dry ice (-70℃)
Component | FC301-01 (100 rxns) |
Calcein-AM Solution(1000×) | 100 ul |
PI Solution(1000×) | 100 ul |