TransStart® KD Plus DNA Polymerase
Referentie AP301-03
Formaat : 6×200units
Merk : TransGen Biotech
TransStart® KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase. This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3’-5’ exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.
Applications
• Fast, high specificity amplification
• High fidelity, high yield amplification
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Component | AP301-01/11 | AP301-02/12 | AP301-03/13 |
TransStart® KD Plus DNA Polymerase | 100 U×1 | 200 U×1 | 200 U×6 |
5×TransStart® KD Plus Buffer | 1.2 ml×1 | 1.2 ml ×2 | 1.2 ml ×12 |
2.5 mM dNTPs | - / 500 μl×1 | - / 1 ml ×1 | - / 1 ml ×6 |
50 mM MgSO4 | 200 μl×1 | 400 μl ×1 | 1 ml ×1 |
6×DNA Loading Buffer | 500 μl×1 | 1 ml ×1 | 1 ml ×2 |
Li P, Du J, Goodier JL, et al. Aicardi–Gouti`eres syndrome protein TREX1 suppresses L1 and maintains genome integrity through exonuclease-independent ORF1p depletion[J]. Nucleic Acids Res. 2017 May 5;45(8):4619-4631.
Zhao K , Du J , Rui Y , et al. Evolutionarily conserved pressure for the existence of distinct G2/M cell cycle arrest and A3H inactivation functions in HIV-1 Vif[J]. Cell Cycle, 2015, 14(6):10.
Xie M , Na‐Na Ren, Yan‐Chun You, et al. Molecular characterisation of two α‐esterase genes involving chlorpyrifos detoxification in the diamondback moth, Plutella xylostella[J]. Pest Management Science, 2017, 73(6):1204.
Wang X , Liu J , Zhu Z , et al. The heparan sulfate-modifying enzyme glucuronyl C5-epimerase HSE-5 controls Caenorhabditis elegans Q neuroblast polarization during migration[J]. Developmental Biology, 2015, 399(2):306-314.
Xie M , Na‐Na Ren, Yan‐Chun You, et al. Molecular characterisation of two α‐esterase genes involving chlorpyrifos detoxification in the diamondback moth, Plutella xylostella[J]. Pest Management Science, 2017, 73(6):1204.