Tri-iodothyronine ELISA Kit (Fish)
Referentie OKCA00348
Formaat : 96Wells
Merk : Aviva Systems Biology
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Telefoonnummer : +1 850 650 7790
| Predicted Species Reactivity | Fish | ||||||||||||||||||||||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Duration | < 4 Hours | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Tri-iodothyronine ELISA Kit (Fish) (OKCA00348) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Fish Tri-iodothyronine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Tri-iodothyronine and incubated. The Tri-iodothyronine found in the sample or standards competes with the biotinylated Tri-iodothyronine for limited binding sites on the immobilized anti-Fish Tri-iodothyronine antibody. Excess unbound biotinylated Tri-iodothyronine and sample or standard Tri-iodothyronine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Tri-iodothyronine captured in the well and inversely proportional to the amount of Tri-iodothyronine which was contained in the sample or standard. | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Range | 0.5 ng/mL - 8 ng/mL | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Reproducibility |
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| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates | ||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | 0.25 ng/ml | ||||||||||||||||||||||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of fish T3. No significant cross-reactivity or interference between fish T3 and analogues was observed. | ||||||||||||||||||||||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA | ||||||||||||||||||||||||||||||||||||||||||
| Gene Symbol | Tri-iodothyronine |
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| Alias Symbols | triiodothyronine, T3, 3,3',5-triiodo-L-thyronine |
| Protein Name | Tri-iodothyronine |
| Description of Target | The L-triiodothyronine (T3, liothyronine) thyroid hormone is normally synthesized and secreted by the thyroid gland in much smaller quantities than L-tetraiodothyronine (T4, levothyroxine, L-thyroxine). Most T3 is derived from peripheral monodeiodination of T4 at the 5 position of the outer ring of the iodothyronine nucleus. The hormone finally delivered and used by the tissues is mainly T3. |