Human VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes such as macrophages. VCAM-1 expression is induced by IL-1beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and Integrin alpha 4 beta 7.||Sample Type:|Cell culture supernatants, serum, plasma, and tissue||Intended Use:|The Human VCAM-1 BioAssay™ ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human VCAM-1 concentrations in cell culture supernatants, serum, plasma, and tissue.||Sensitivity:|60pg/ml||Range:|125-8000pg/ml||Specificity:|Recognizes human VCAM-1.||Sample Volume:|100ul/well||Test Principle:|This assay employs the Sandwich immunoassay technique. An anti-h VCAM-1 monoclonal coating antibody is adsorbed onto microwells. VCAM-1 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h VCAM-1 antibody is added and binds to VCAM-1 captured by the first antibody. Following incubation unbound Biotinylated anti-h VCAM-1 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h VCAM-1 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of VCAM-1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven VCAM-1 standard dilutions and VCAM-1 sample concentration determined.||Kit Components:|Aluminum pouches with a Microwell Plate: 8x6|Standard: 1xVial|Biotinylated antibody: 1xVial|Streptavidin-HRP: 1xVial|Assay Buffer (25ml/Vial): 1xVial|Wash Buffer Concentrate 20x (30ml/Vial): 1xVial|TMB Substrate Solution: 1x6ml|Stop Solution (12ml/Vial): 1xVial|Plate Covers- Adhesive strips: 2xstrips|Instruction: 1x||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.