Vegfc Rabbit Polyclonal Antibody

CAT#: DP3507S

Vegfc rabbit polyclonal antibody, Azide Free

Size: 100 ug 200 ug


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Specifications

Product Data
Applications ELISA, IP, WB
Recommended Dilution ELISA: 2.5 µg/ml, allows the detection of 0.5-1.0 ng/well of recombinant rat VEGF-C. 
Western Blot: 2-5 μg/ml.
Immunoprecipitation: 1-10 µg / ml lysate or reaction volume.
Reactivities Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen Recombinant Rat VEGF-C native protein.(Asp101-Ile221) derived from insect cells (Cat.-No DA3519X)
Specificity The antibody will react with Rat VEGF-C and will cross-react with Human and Mouse VEGF-C proteins.
Formulation PBS, pH 7.4, containing no preservative.
State: Azide Free
State: Lyophilized purified Ig fraction.
Reconstitution Method Restore in sterile water to a concentration of > 0.5 mg/ml.
Purification Protein A affinity chromatography.
Conjugation Unconjugated
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name vascular endothelial growth factor C
Background VEGF-C, a member of the VEGF/PDGF family of structurally related proteins, is a potent angiogenic cytokine. It promotes endothelial cell growth, promotes lymphangiogesis, and can also affect vascular permeability. VEGF-C is expressed in various tissues, but is not produced in peripheral blood lymphocytes. It forms cell surfaced-associated non-covalent disulfide linked homodimers, and can bind and activate both VEGFR-2 (flk1) and VEGFR-3 (flt4) receptors. During embryogenesis, VEGF-C may play a role in the formation of the venous and lymphatic vascular systems. Both VEGF-C and VEGF-D are over-expressed in certain cancers, and the resulting elevated levels of VEGF-C or VEGF-D tend to correlate with increased lymphatic metastasis. Recombinant, fully processed rat VEGF-C is a 16-18 kDa non-disulfide linked homodimeric protein consisting of two 116 amino acid polypeptide chains. Due to glycosylation the protein migrates as a 20.0-22.0 kDa band under non-reducing condition.
Synonyms VEGFC, VRP, Vascular endothelial growth factor C, Vascular endothelial growth factor-related protein, Flt4 ligand
Reference Data

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