14-3-3, alpha, beta (Protein 1054, Protein Kinase C Inhibitor Protein 1, brain protein 14-3-3, beta Isoform, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide, protein kinase C inhibitor protein-1) (APC)

Référence 0014-02-APC-100ul

Conditionnement : 100ul

Marque : US Biological

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0014-02-APC 14-3-3, alpha, beta (Protein 1054, Protein Kinase C Inhibitor Protein 1, brain protein 14-3-3, beta Isoform, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide, protein kinase C inhibitor protein-1) (APC)

Clone Type
Polyclonal
Host
rabbit
Source
human
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA WB
Crossreactivity
Bo Ce Hu Mo Rt Xe
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 expression is particularly high in certain neural tissues and studies in Drosophila have established a role for 14-3-3 proteins in nervous system development and function. Recently, a dimeric 14-3-3 protein was determined to be an essential cofactor for Raf kinase activity.||Applications: |Suitable for use in FLISA and Western Blot. Other applications not tested.||Recommended Dilutions:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by immunoaffinity chromatography.|Immunogen: Synthetic peptide corresponding to 20aa from the N-terminus of the human 14-3-3 beta/alpha protein. Sequence Homology: delta and zeta isoforms: single aa change; tau isoform: three aa change; gamma isoform: four aa change. |Specificity: Recognizes human and is broadly reactive with members of the 14-3-3 family of proteins (~29–32kD). Species Crossreactivity: bovine, C. Elegans, mouse, rat and Xenopus. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic peptide corresponding to 20aa from the N-terminus of the human 14-3-3 beta/alpha protein. Sequence Homology: delta and zeta isoforms: single aa change; tau isoform: three aa change; gamma isoform: four aa change.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human and is broadly reactive with members of the 14-3-3 family of proteins (~29–32kD). Species Crossreactivity: bovine, C. Elegans, mouse, rat and Xenopus.
References
1. Skoulakis, E.M., and Davis, R.L., Mol. Neurobiol. 16:269:284 (1998). 2. Tzivion, G., et al., Nature 394:88–92 (1998).