2×TransStart® FastPfu PCR SuperMix (-dye)
Référence AS221-01
Conditionnement : 1ml
Marque : TransGen Biotech
TransStart® FastPfu PCR SuperMix is a ready-to-use mixture of TransStart® FastPfu DNA polymerase, dNTPs, and optimized buffer, featuring high amplification efficiency, fast amplification speed, high fidelity and high specificity. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2 O for amplification. The amplified product of 2×TransStart® FastPfu PCR SuperMix is blunt-ended and can be cloned directly into pEASY®-Blunt series of vectors. It can also be directly loaded on agarose gel for electrophoresis. If it is used for cloning, it needs to be purified to remove the dye.
Its PCR product is not suitable for polyacrylamide gel electrophoresis.
• Reduce PCR operation time.
• Avoid contamination caused by the multi-step operation.
• TransStart® FastPfu PCR SuperMix offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.
Applications
• High fidelity PCR
• Site-directed mutagenesis
• Blunt end cloning
• Complex templates
• Amplification of templates with high GC/AT content
• Long fragment amplification
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Component | AS221-01 | AS221-02 |
2×TransStart® FastPfu PCR SuperMix (-dye)/ (+dye) | 1 ml | 5×1 ml |
Nuclease-free Water | 1 ml | 5 ml |
Cheng S , Li C , Wang Y , et al. Characterization and expression analysis of a thioredoxin-like protein gene in the sea cucumber Apostichopus japonicus[J].
Fish & Shellfish Immunology, 2016:S1050464816305459.
Zhang, Yan‐Jun, Xie M , Zhang, Xiao‐Lin, et al. Establishment of polyethylene‐glycol‐mediated protoplast transformation for Lecanicillium lecanii and development of virulence‐enhanced strains against Aphis gossypii[J]. Pest Management Science, 2016, 72(10):8.
Wang Y , Gao X , Yao Y , et al. The dynamics of Chinese variant porcine epidemic diarrhea virus production in Vero cells and intestines of 2-day old piglets.[J]. Virus Research, 2015, 208:82-88.
Wang S , Cong H , Kong L , et al. Complete mitochondrial genome of the greater bandicoot rat Bandicota indica (Rodentia: Muridae)[J]. DNA Sequence, 2016, 27(6):2.
Han L S , Huang Z , Han F , et al. Eight novel MUT loss-of-function missense mutations in Chinese patients with isolated methylmalonic academia[J]. World Journal of Pediatrics, 2017, 13(4):381-386.