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> Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) (AAT/1378), 0.2mg/mL

Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) (AAT/1378), 0.2mg/mL

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Référence BNUB1378-500

Conditionnement : 500uL

Marque : Biotium

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Alpha-1 Antitrypsin Monoclonal Mouse Antibody (AAT/1378)

Product Attributes

research-areas

Cancer, Inflammation
Antibody number

#1378
Antibody reactivity (target)

Alpha-1 Antitrypsin
Antibody type

Primary
Host species

Mouse
Clonality

Monoclonal
Clone

AAT/1378
Isotype

IgG1
Molecular weight

54 kDa
Synonyms

Alpha-1 antitrypisin; SERPINA1; Alpha-1 protease inhibitor; Alpha-1 antiproteinase; AAT; Serpin A1
Human gene symbol

SERPINA1
Entrez gene ID

5265
SwissProt

P01009
Unigene

525557
Immunogen

Recombinant human Alpha-1-Antitrypsin (AAT) protein fragment (exact sequence is proprietary)
Verified antibody applications

IHC (FFPE) (verified), WB (verified)
Antibody target cellular localization

Secreted (extracellular)
Species reactivity

Human
Antibody application notes

Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min, Immunofluorescence 0.5-1 ug/mL Western blotting 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Western blotting 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user
Positive control

HepG2 Cells. Tonsil, Hepatocellular Carcinoma or Histiocytoma.
Shipping condition

Room temperature
Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status

For research use only (RUO)
Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Antibody research areas

Cancer, Inflammation
Product origin

Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Tumor expression

Liver cancer

Technology

CF® Dyes
Considerations for Immunofluorescence Staining
Primary & Secondary Antibody Conjugates
Protocols
SDS
Supporting Documents
Download Datasheet

Product Description

This antibody recognizes a protein of 54 kDa, which is identified as Alpha-1 antitrypsin (AAT). AAT is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email info@clinisciences.com to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 1378, Anti-Alpha-1 Antitrypsin (AAT/1378) 

Antibody # prefix Conjugation Ex/Em (nm) Laser line Detection channel Dye Features
BNC04 CF®405S 404/431 405 DAPI (microscopy), AF405 CF®405S Features
BNC88 CF®488A 490/515 488 GFP, FITC CF®488A Features
BNC68 CF®568 562/583 532, 561 RFP, TRITC CF®568 Features
BNC94 CF®594 593/614 561 Texas Red® CF®594 Features
BNC40 CF®640R 642/662 633-640 Cy®5 CF®640R Features
BNC47 CF®647 650/665 633-640 Cy®5 CF®647 Features
BNC74 CF®740 742/767 633-685 775/50 CF®740 Features
BNCB Biotin N/A N/A N/A
BNUB Purified N/A N/A N/A
BNUM Purified,
BSA-free		 N/A N/A N/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

Note: Listed references are for this antibody clone sold by Biotium and other suppliers.