Dynamic Circulation and Perfusion of Cell Culture Medium Within a Multi-welled Plate

Alvetex Perfusion Plates are a simple, low-cost perfusion system that can combine 3D cell culture with dynamic circulation and perfusion of cell culture media. This product allows scientists to create cell based models that are another step closer to the environment experienced by cells and tissue in vivo. The systems can also be used to create complex co-cultures, multi organ systems and to study paracrine effects.

The Alvetex perfusion system is compatible with the following methods:

• 3D cell culture using Alvetex well insert technology
• 3D co-culture using Alvetex well insert technology
• Standard 2D cell culture
• Hydrogel-based 3D cell cultures
• Transwell and well insert technology from various suppliers

About Alvetex

• Alvetex is a highly porous polystyrene scaffold designed for 3D cell culture
  Cells grown in Alvetex possess a natural tissue-like structure that enables them to function in a more physiologically relevant manner. Alvetex 3D cell culture enables cells maintain their in vivo morphology, behavior and responsiveness within an in vitro model system.
• Alvetex is a unique cellular environment
  Manufactured to the highest standards of consistency each disc is engineered to a thickness of just 200 µm with pore sizes of 36-40 µm (Alvetex Scaffold). No cell is ever further than 100 µm from the nutrient source enabling easily exchange of nutrients, gases and waste products by passive diffusion across short distances.
• Maintain natural cell shape and morphology
  In conventional 2D cell culture, cells come into contact with the flat surface of the culture vessel (e.g. Petri dish, flask or multi-well plate). In this unnatural environment cells become flattened against the substrate. In this abnormally thin structure there has been significant re-modelling of the internal cellular components. The entire cytoskeleton is remodeled and organelles such as the nucleus are flattened.

• Alvetex has been designed for simple and routine use
  It uses conventional cell culture plasticware. Therefore any cell biologist can get into Alvetex 3D cell culture.

  • No specialist equipment is required
  • No changes to media and optimized cell feeding protocols
  • Compatible with the majority of down stream analytical techniques.

Say goodbye to risks of foreign unknown materials such as proteins or cytokines of animal origin — unlike conventional materials used in cell culture Alvetex is made from polystyrene and is completely inert.

Available in varying formats and sizes.

See Alvetex Publications.