Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC-P 1:100-300 ELISA 1:20000 IF 1:100-300
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	FAP
Gene ID:	2191
Uniprot ID:	SEPR_HUMAN
Immunogen Region:	331-380 aa
Specificity:	Seprase Polyclonal Antibody detects endogenous levels of Seprase protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human FAP-1 at the amino acid range 331-380

Post Translational Modifications	N-glycosylated. The N-terminus may be blocked.
Function	Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2. Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vitronectin, tenascin, laminin, fibronectin, fibrin or casein. Also has dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.
Protein Name	Prolyl Endopeptidase Fap 170 Kda Melanoma Membrane-Bound Gelatinase Dipeptidyl Peptidase Fap Fibroblast Activation Protein Alpha Fapalpha Gelatine Degradation Protease Fap Integral Membrane Serine Protease Post-Proline Cleaving Enzyme Serine Integral Membrane Protease Simp Surface-Expressed Protease Seprase Cleaved Into - Antiplasmin-Cleaving Enzyme Fap - Soluble Form Apce
Cellular Localisation	Prolyl Endopeptidase Fap: Cell Surface Cell Membrane Single-Pass Type Ii Membrane Protein Cell Projection Lamellipodium Membrane Invadopodium Membrane Ruffle Membrane Membrane Localized On Cell Surface With Lamellipodia And Invadopodia Membranes And On Shed Vesicles Colocalized With Dpp4 At Invadopodia And Lamellipodia Membranes Of Migratory Activated Endothelial Cells In Collagenous Matrix Colocalized With Dpp4 On Endothelial Cells Of Capillary-Like Microvessels But Not Large Vessels Within Invasive Breast Ductal Carcinoma Anchored And Enriched Preferentially By Integrin Alpha-3/Beta-1 At Invadopodia Plasma Membrane Protrusions That Correspond To Sites Of Cell Invasion In A Collagen-Dependent Manner Localized At Plasma And Ruffle Membranes In A Collagen-Independent Manner Colocalized With Plaur Preferentially At The Cell Surface Of Invadopodia Membranes In A Cytoskeleton- Integrin- And Vitronectin-Dependent Manner Concentrated At Invadopodia Membranes Specialized Protrusions Of The Ventral Plasma Membrane In A Fibrobectin-Dependent Manner Colocalizes With Extracellular Components (Ecm) Such As Collagen Fibers And Fibronectin Antiplasmin-Cleaving Enzyme Fap Soluble Form: Secreted Found In Blood Plasma And Serum Isoform 2: Cytoplasm
Alternative Antibody Names	Anti-Prolyl Endopeptidase Fap antibody Anti-170 Kda Melanoma Membrane-Bound Gelatinase antibody Anti-Dipeptidyl Peptidase Fap antibody Anti-Fibroblast Activation Protein Alpha antibody Anti-Fapalpha antibody Anti-Gelatine Degradation Protease Fap antibody Anti-Integral Membrane Serine Protease antibody Anti-Post-Proline Cleaving Enzyme antibody Anti-Serine Integral Membrane Protease antibody Anti-Simp antibody Anti-Surface-Expressed Protease antibody Anti-Seprase Cleaved Into - Antiplasmin-Cleaving Enzyme Fap - Soluble Form antibody Anti-Apce antibody Anti-FAP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance