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> Anti-SARS-CoV-2-Spike S2 ECD antibody (686-1214)

Anti-SARS-CoV-2-Spike S2 ECD antibody (686-1214)

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Référence NB-22-72380-100

Conditionnement : 100uL

Marque : Neo Biotech

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  • Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g SARS-CoV-2 Spike S2 ECD antibody . Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike S2 ECD antibody at a dilution of 1:5000.
  • Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 Spike S2 ECD fusion protein (top left) and untreated 293T cells (top right) use SARS-CoV-2 Spike S2 ECD Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immobilized Recombinant SARS-COV2 S-ECD-PFS Protein (RP01283LQ) at 1 Mu g/mL (100 Mu L/well) can bind SARS-CoV-2 Spike S2 ECD Rabbit polyclonal antibody with a linear range of 3. 12-50ng/mL.
  • Western blot analysis of extracts of normal 293T cells 293T transfected with Spike S2 ECD Protein, using SARS-CoV-2 Spike S2 ECD Rabbit polyclonal antibody at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 1s.

General Info

Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SARS-CoV-2-Spike S2 ECD (686-1214) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: DB 1:500-1:2000
WB 1:2000-1:6000
IF/ICC 1:50-1:200
IP 1:2000-1:6000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Immunogen Region: 686-1214
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 686-1214 of coronavirus Spike S2 ECD (YP_009724390.1).
Immunogen Sequence: SVASQSIIAYTMSLGAENSV AYSNNSIAIPTNFTISVTTE ILPVSMTKTSVDCTMYICGD STECSNLLLQYGSFCTQLNR ALTGIAVEQDKNTQEVFAQV KQIYKTPPIKDFGGFNFSQI LPDPSKPSKRSFIEDLLFNK VTLADAGFIKQYGDCLGDIA ARDLICAQKFNGLTVLPPLL TDEMIAQYTSALLAGTITSG WTFGAGAALQIPFAMQMAYR FNGIGVTQNVLYENQKLIA

Description

Background This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is thought to be the predominant enzyme responsible for epoxidation of endogenous arachidonic acid in cardiac tissue. Multiple transcript variants have been found for this gene.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance