ARCA EGFP mRNA
Référence R1001-100ug
Conditionnement : 100ug(1mg/mL)
Marque : APExBIO Technology
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
ARCA EGFP mRNA
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
EGFP (enhanced green fluorescent protein) mRNA can express an enhanced green fluorescence once has been transfected into cells, which is a direct-detection reporter mRNA, used as control to study transfection and expression in mammalian cells by various fluorescence-based assays. You can observe the fluorescence at 509 nm.
Our product had already been modified with ARCA with a high efficient co-transcriptional capping method, which means most of mRNA has a Cap 0 structure. It is a more mature mRNA than mRNA with no capping and maintain a more stable state which lead to higher expressing efficiency in transfected mammalian cells. ARCA obtain the ability only inserting in the proper orientation, results in forming mRNAs that can be translated twice efficiently as those initiated with mCAP.
A upgraded version of ARCA EGFP mRNA is available, please check out R1007 ARCA EGFP MRNA (5-moUTP) for more details.
- 1. Peiying Li, et al. "Targeted mRNA Nanoparticles Ameliorate Blood–Brain Barrier Disruption Postischemic Stroke by Modulating Microglia Polarization." ACS Nano. 2024 Jan 30;18(4):3260-3275. PMID: 38227975
- 2. Yan Liang, Jingge Zhang, et al. "Biomimetic Mineralized CRISPR/Cas RNA Nanoparticles for Efficient Tumor-Specific Multiplex Gene Editing." ACS Nano. 2023 Aug 8;17(15):15025-15043. PMID: 37481734
- 3. Hao Chen, Lina Guo, et al. "A General and Efficient Strategy for Gene Delivery Based on Tea Polyphenols Intercalation and Self‐Polymerization." Adv Sci (Weinh). 2023 Aug;10(24):e2302620. PMID: 37349886
- 4. Qiming Yin, Xiang Song, et al. "Incorporation of glycyrrhizic acid and polyene phosphatidylcholine in lipid nanoparticles ameliorates acute liver injury via delivering p65 siRNA." Nanomedicine. 2022 Dec 27;48:102649. PMID: 36584740
Identity & Purity
- Agarose Gel Mobility: Pass.
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QC - MSDS (Material Safety Data Sheet)
Concentration ± 6%: Pass.