Western blot of 293T cells transfected (+) or untrans-fected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using Biotin Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146, at 0.2 µg/mL, followed by a HRP conjugated Streptavidin. Immunohistochemistry staining of naïve HepG2 cells (Negative control) using anti-His-Tag antibody, Clone RM146. Immunohistochemistry staining of 293T cells expressing His-Tag nuclear protein X, using anti-His-Tag antibody, Clone RM146.
Product Details Product Name Biotin Anti-His-Tag (6xHis-Tag or 10xHis-Tag) Rabbit Monoclonal Antibody, Clone RM146 Product Description Biotinylated Rabbit monoclonal to His-Tag. (6xHis-Tag or 10xHis-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy) Catalog No. 31-1048-02 Clone Name RM146 Product Data Sheet Biotin Anti-His Tag Rabbit Monoclonal Antibody Data Sheet RM146 Specificity RM146 reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells. Immunogen Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus. Application Western Blot
ELISA
Immunoprecipitation
Immunocytochemistry
Flow Cytometry
Immunohistochemistry Species Reactivity All Conjugate Biotin Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Size 50 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage Western Blot: 0.1 µg/mL – 0.5 µg/mL;
Immunocytochemistry 0.5 µg/mL – 2 µg/mL;
Flow Cytometry: 0.5 µg/mL – 2 µg/mL;
Immunohistochemistry; 0.1 µg/mL - 1 µg/mL;
ELISA: 0.01 µg/mL-0.5 µg/mL. Keywords . Product Images 
A titer ELISA performed on purified His-Tag-PCSK9 recombinant protein. A serial dilution of Biotin Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146, was used as the primary antibody, followed by alkaline phosphatase conjugated Streptavidin. 
Biotin Anti-His-Tag rabbit monoclonal antibody [RM146]
![Biotin Anti-His-Tag rabbit monoclonal antibody [RM146]](https://www.clinisciences.com/upload/-img-lapin1.png)
Référence 31-1048-02
Conditionnement : 50ug
Marque : RevMab Biosciences
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![Biotin Anti-His-Tag Rabbit Monoclonal Antibody [Clone RM146]; 6x His-Tag or 10x His-Tag; 31-1048-02](/proxy_img/aHR0cHM6Ly93d3cucmV2bWFiLmNvbS93cC1jb250ZW50L3VwbG9hZHMvMjAxNi8xMC9GaWcyX1dCX0Jpb3Rpbi1SYnRYSGlzVGFnXzIucG5n.png)
![Biotin Anti-His-Tag Rabbit Monoclonal Antibody [Clone RM146]; 6x His-Tag or 10x His-Tag; 31-1048-02 - Image 2](/proxy_img/aHR0cHM6Ly93d3cucmV2bWFiLmNvbS93cC1jb250ZW50L3VwbG9hZHMvMjAxNS8xMS9LMzZNX0hpc3RhZ19JSEMucG5n.png)
![Biotin Anti-His-Tag Rabbit Monoclonal Antibody [Clone RM146]; 6x His-Tag or 10x His-Tag; 31-1048-02 - Image 3](/proxy_img/aHR0cHM6Ly93d3cucmV2bWFiLmNvbS93cC1jb250ZW50L3VwbG9hZHMvMjAxNS8xMS9IZXBHMl9IaXN0YWdfSUhDLnBuZw==.png)
![Biotin Anti-His-Tag Rabbit Monoclonal Antibody [Clone RM146]; 6x His-Tag or 10x His-Tag; 31-1048-02 - Image 4](/proxy_img/aHR0cHM6Ly93d3cucmV2bWFiLmNvbS93cC1jb250ZW50L3VwbG9hZHMvMjAxNi8xMC9GaWcxX0VMSVNBX0Jpb3Rpbi1SYnRYSGlzVGFnXzEucG5n.png)
Biotinylated Rabbit monoclonal to His-Tag (6x His-Tag or 10x His-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy)
50 µg