CA125 ELISA Kit (Mouse)
Référence OKCD04366
Conditionnement : 96Wells
Marque : Aviva Systems Biology
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| Datasheets/Manuals | Printable datasheet for CA125 ELISA Kit (Mouse) (OKCD04366) |
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| Predicted Species Reactivity | Mouse | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbohydrate Antigen 125 (CA125). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbohydrate Antigen 125 (CA125). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbohydrate Antigen 125 (CA125), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carbohydrate Antigen 125 (CA125) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbohydrate Antigen 125 (CA125) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbohydrate Antigen 125 (CA125) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 6.5 pg/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | CA125 |
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| Protein Name | Carbohydrate Antigen 125 |
| Uniprot ID | Q9D1H1 |