Caspase 8 (CASP8) (180-480) Mouse Monoclonal Antibody [Clone ID: 5D3]
CAT#: AM26607AF-N
Caspase 8 (CASP8) (180-480) mouse monoclonal antibody, clone 5D3, Azide Free
Specifications
Product Data | |
Clone Name | 5D3 |
Applications | IP, WB |
Recommended Dilution | Western blot: 1 µg/ml for chemiluminescence detection system. Immunoprecipitation: 2 µg/500 µl of cell extract. For details see protocol below. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG2b |
Clonality | Monoclonal |
Immunogen | Recombinant human caspase s-8 corresponding to C-terminal amino acids (180-480 aa) |
Specificity | This antibody reacts with caspase-8. |
Formulation | PBS containing 50% glycerol, pH 7.2. No preservative is contained. State: Azide Free State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein A agarose |
Conjugation | Unconjugated |
Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Gene Name | caspase 8 |
Database Link | |
Background | Caspase-8 (FLICE/MACH/Mch5) is a member of the ICE (interleukin- 1 converting enzyme)/CED-3 family cysteine protease. It is the most upstream protease that recei ves the activation signal from the Fas (APO1/CD95) and TNFR1 (Tumor Necrosis Factor Receptor 1) to initiate the ap optotic protease cascade that leads to activation of ICE/CED-3 family proteases. Caspase-8 has high homologous region to the ICE/CED-3 family in C-terminal and two death effecter domains (DED) in N-terminal. Binding of caspase-8 to FADD (MORT1) through association of their DED, and consequent activation of the caspases by th eir proteolytic cleavage, are thought to be critical steps in the initiation of Fas- and TNFR1-induced apoptosis. Recen tly the inhibitor of Fas- and TNFR1-induced apoptosis is identified, called I-FLICE (FLIP/Casper/FLAME/CASH). I-FLICE has high homology to caspase-8 and it contains two DED, which interacts with caspase-8 and FADD, and potently inhibits Fas- and TNFR1-induced apoptosis. |
Synonyms | CASP-8, CASP8, MCH5, CAP4 |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blotting Immunoprecipitation |
Reference Data |
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