CD71 / Transferrin Receptor (TFRC) (Extracellular Domain) (TFRC/1818), Biotin conjugate, 0.1mg/mL
Référence BNCB1818-500
Conditionnement : 500uL
Marque : Biotium
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Téléphone : +1 850 650 7790
| Antibody number | #1818 |
|---|---|
| Antibody reactivity (target) | CD71, Transferrin Receptor |
| Antibody type | Primary |
| Host species | Mouse |
| Clonality | Monoclonal |
| Clone | TFRC/1818 |
| Isotype | IgG2b, kappa |
| Molecular weight | 85-95 kDa (monomer); 190 kDa (dimer) |
| Synonyms | Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR |
| Human gene symbol | TFRC |
| Entrez gene ID | 7037 |
| SwissProt | P02786 |
| Unigene | 529618 |
| Immunogen | Recombinant human TFRC protein fragment (aa94-212) (exact sequence is proprietary) |
| Antibody target cellular localization | Plasma membrane |
| Verified antibody applications | IHC (FFPE) (verified) |
| Species reactivity | Human |
| Antibody application notes | Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 1-2 ug/mL, ELISA: 2-4 ug/mL for coating order Ab without BSA, Flow cytometry: 0.5-1 ug/million cells in 0.1mL, Immunohistology (formalin) 1-2 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min, Western blotting 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user |
| Positive control | PC3 cells. Human placenta or breast carcinoma. |
| Shipping condition | Room temperature |
| Storage Conditions | Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C |
| Shelf life | Guaranteed for at least 24 months from date of receipt when stored as recommended |
| Regulatory status | For research use only (RUO) |
| Antibody/conjugate formulation | Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide |
| Cell/tissue expression | Microvascular endothelial cells |
| Antibody research areas | Cancer |
| Product origin | Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot. |
Product Description
This antibody recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Catalog number key for antibody number 1818, Anti-CD71 (TFRC/1818)
| Antibody # prefix | Conjugation | Ex/Em (nm) | Laser line | Detection channel | Dye Features |
|---|---|---|---|---|---|
| BNC04 | CF®405S | 404/431 | 405 | DAPI (microscopy), AF405 | CF®405S Features |
| BNC88 | CF®488A | 490/515 | 488 | GFP, FITC | CF®488A Features |
| BNC68 | CF®568 | 562/583 | 532, 561 | RFP, TRITC | CF®568 Features |
| BNC94 | CF®594 | 593/614 | 561 | Texas Red® | CF®594 Features |
| BNC40 | CF®640R | 642/662 | 633-640 | Cy®5 | CF®640R Features |
| BNC47 | CF®647 | 650/665 | 633-640 | Cy®5 | CF®647 Features |
| BNC74 | CF®740 | 742/767 | 633-685 | 775/50 | CF®740 Features |
| BNCB | Biotin | N/A | N/A | N/A | |
| BNUB | Purified | N/A | N/A | N/A | |
| BNUM | Purified, BSA-free | N/A | N/A | N/A |
Note: Listed references are for this antibody clone sold by Biotium and other suppliers.
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