Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

Danicamtiv [1970972-74-7]

Imprimer

Référence HY-109128-1ml

Conditionnement : 10mM/1mL

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Marque : MedChemExpress

Contactez votre distributeur local :

Téléphone : +1 850 650 7790

Danicamtiv (MYK-491), an inotropic agent, is a selective allosteric activator of cardiac myosin. Danicamtiv increases cardiac systolic function and preserves mechanical efficiency.

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Danicamtiv Chemical Structure

CAS No. : 1970972-74-7

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Description

Danicamtiv (MYK-491), an inotropic agent, is a selective allosteric activator of cardiac myosin. Danicamtiv increases cardiac systolic function and preserves mechanical efficiency^[1].

In Vivo

Danicamtiv selectively enhances cardiac actomyosin activity, the molecular force‐generating unit of the sarcomere, prolonging contraction while preserving actin–myosin detachment, allowing relaxation, and without impacting Ca²⁺ homeostasis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Essai clinique

Masse moléculaire

435.42

Formule

C₁₆H₂₀F₃N₅O₄S

CAS No.

1970972-74-7

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=S([C@@](C1CCN(CC1)C(NC2=NOC=C2)=O)(C)F)(C3=CN(N=C3C(F)F)C)=O

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvant et solubilité

In Vitro:

DMSO : 50 mg/mL (114.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2966 mL	11.4832 mL	22.9663 mL
5 mM	0.4593 mL	2.2966 mL	4.5933 mL
10 mM	0.2297 mL	1.1483 mL	2.2966 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureté et documentation

Purity: 99.12%

Select Batch:

Fiche technique (273 KB) SDS (252 KB)

COA (250 KB) LCMS (257 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Sarah Fernandes, et al. Abstract 15707: MYK-491, a Novel Small-Molecule Cardiac Myosin Activator Increases Cardiac Systolic Function and Preserves Mechanical Efficiency: Pre-Clinical in vivo and in vitro Evidence. Circulation. 2019;140:A15707

[2]. Voors AA, et al. Effects of danicamtiv, a novel cardiac myosin activator, in heart failure with reduced ejection fraction: experimental data and clinical results from a phase 2a trial. Eur J Heart Fail. 2020;22(9):1649-1658. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2966 mL	11.4832 mL	22.9663 mL	57.4158 mL
	5 mM	0.4593 mL	2.2966 mL	4.5933 mL	11.4832 mL
	10 mM	0.2297 mL	1.1483 mL	2.2966 mL	5.7416 mL
	15 mM	0.1531 mL	0.7655 mL	1.5311 mL	3.8277 mL
	20 mM	0.1148 mL	0.5742 mL	1.1483 mL	2.8708 mL
	25 mM	0.0919 mL	0.4593 mL	0.9187 mL	2.2966 mL
	30 mM	0.0766 mL	0.3828 mL	0.7655 mL	1.9139 mL
	40 mM	0.0574 mL	0.2871 mL	0.5742 mL	1.4354 mL
	50 mM	0.0459 mL	0.2297 mL	0.4593 mL	1.1483 mL
	60 mM	0.0383 mL	0.1914 mL	0.3828 mL	0.9569 mL
	80 mM	0.0287 mL	0.1435 mL	0.2871 mL	0.7177 mL
	100 mM	0.0230 mL	0.1148 mL	0.2297 mL	0.5742 mL

Danicamtiv Related Classifications

Cytoskeleton
Myosin

Danicamtiv [1970972-74-7]

Référence HY-109128-1ml

Contactez votre distributeur local :

Marque : MedChemExpress

Contactez votre distributeur local :

Calculateur de molarité

Calculateur de dilution

Complete Stock Solution Preparation Table

Danicamtiv Related Classifications

Keywords:

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