Dolichos biflorus (DBA)

Dolichos biflorus agglutinin (DBA) is isolated from horse gram and purified by affinity chromatography. DBA is a glycoprotein with a molecular weight of about 111,000 and consists of 4 subunits of equal size. This lectin has an isoelectric point between pH 4.6 and pH 5.5. It has a carbohydrate specificity toward alpha-N-acetylgalactosamine and elutes with the sugar GalNAc. DBA has been used as a general marker of developing renal collecting ducts, which can then be used to establish secretion status in blood group A individuals by hemagglutination inhibition and for detecting blood typing. DBA is over thousand times more active in agglutinating blood group A1 cells versus A2 cells, and is used to distinguish these blood types in routine serology applications, and has potential applications in examining tumors which affect the levels of blood group substances. Calcium, manganese, magnesium, and zinc ions are required for binding.||Source:|Horse gram||Blood Specificity:|A1 >> A2||Sugar Specificity:|alpha-N-Acetylgalactosamine||Inhibitory Carbohydrate:|N-Acetylgalactosamine||Divalent Ions:|Ca++, Mn++, Mg++, Zn++||Solubility:|Soluble in 0.01M Phosphate Buffered Saline||Mitogenic Activity:|No||Applications: |Suitable for use in Glycobiology, Blotting and Immunohistochemistry. Other applications not tested. ||Storage and Stability:|Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.