Anti-Donkey IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Donkey IgG and Donkey Serum.
Conjugation
Biotin
Storage
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives
0.01% (w/v) Sodium Azide
Alternative names
goat anti-Donkey IgG Antibody biotin conjugation,
Note
For research use only
Application notes
Anti-Donkey IgG (H&L) has been assayed against 1.0 ug of Donkey IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:160,000 of the reconstitution concentration is suggested for this product. Specific conditions for reactivity should be optimized by the end user.