G418 Sulfate (Geneticin®)

Référence G1000-25g

Conditionnement : 25g

Marque : US Biological

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G1000 G418 Sulfate (Geneticin®)

Clone Type
Polyclonal
Grade
Molecular Biology Grade
Shipping Temp
RT
Storage Temp
4°C

G418 Sulfate is an aminoglycoside related to Gentamycin, used as a selective agent in bacterial genetics. It is toxic to yeast, bacteria, many plant and mammalian cells, helminths and protozoans.||Synonyms:|O-2-Amino-2,7-dideoxy-D-glycero-α-D-gluco-heptopyranosyl-(1→4)-O-[3-deoxy-4-C-methyl-3-(methylamino)-β-L-arabinopyranosyl-(1→6)]-2-deoxy-D-streptamine Sulfate (1:2); Antibiotic G 418 Sulfate; G418 Sulfate||CAS No:|108321-42-2||Molecular Formula:|C20H40N4O10•2H2SO4||Molecular Weight:|692.71||Appearance:|White, crystalline powder||Solubility:|Colorless, clear, complete||Potency:|≥620ug/mg||Suggested Working Concentrations:|100-800ug/ml|Mammalian Selection: 400ug/ml|Maintenance: 200ug/ml||Absorbance (1mg/ml):|280nm: ≤0.015|570nm: ≤0.10||Water (KF):|≤12%||Specific Rotation:|+104° to +121ºC||Reconstitution:|Reconstitute with water or ethanol to a desired stock concentration. Sterilize by filtration through a 0.2um filter, aliquot and freeze at -20°C.||Storage and Stability:|Powder: Store at 4ºC. Avoid moisture uptake, as this can decrease potency. Stable for 12 months after receipt.|Stock solution: Aliquot and store at -20°C. Stable for 3-6 months -at 20°C.

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.
References
1. Fatholahi, M. et al., (2006) J. Pharm. and Exp. Therapeutics 317:676-684. 2. Upadhyay, A.K. and Edmondson, D.E. (2008) Protein Expr Purif. 59:349-56. 3. Veranicˇ, P. et al., (2008) Biophysical J. 95:4416–4425. 1. Ausubel, F. M., et al., “Current Protocols in Molecular Biology”, John Wiley, ed. (1992) 2. Maniatis, T., et al., “Molecular Cloning: A Laboratory Manual”, Cold Spring Harbor Laboratory Press, ed. (1989)

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