Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> Goat anti-VE-cadherin

Goat anti-VE-cadherin

Imprimer Imprimer
Identifiez-vous

Référence EB12667

Conditionnement : 100ug

Marque : Everest Biotech

Demander plus d'informations

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

Code Name Applications Availability Size Tested species Grade
EB12667 Goat Anti-VE-cadherin Antibody Pep-ELISA, IF, FC Yes 100
EB12667 1
EB12667 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB12667 2
EB12667 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Target Protein

Principal Names: CDH5, cadherin 5, type 2 (vascular endothelium), 7B4, CD144, 7B4 antigen, VE-cadherin, cadherin 5, type 2, VE-cadherin (vascular epithelium), cadherin-5, cd144 antigen, endothelial-specific cadherin, vascular endothelial cadherin
Official Symbol: CDH5
Accession Number(s): NP_001786.2
Human GeneID(s): 1003

Immunogen

Peptide with sequence C-KQARAHGKSVPE, from the internal region of the protein sequence according to NP_001786.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20>Applications Tested Peptide ELISA: antibody detection limit dilution 1:128000.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Pig, Cow