IL2 Mouse Monoclonal Antibody [Clone ID: 1H10-1-1]
Référence PM1000A
Conditionnement : 500ug
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| Product Data | |
| Clone Name | 1H10-1-1 |
|---|---|
| Application | ELISA, IHC, WB |
| Application | Sandwich ELISA: In a Sandwich ELISA (assuming 100 µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human IL-2 when used with Biotin conjugated anti-Human IL-2 (Cat.-No PP1024B) as the detection antibody at a concentration of approximately 1.0-2.0 µg/ml. Western Blot: To detect human IL-2 by Western blot analysis this antibody can be used at a concentration of 1.0-2.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions. Immunohistochemistry on Paraffin Sections: 2.5-5.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium citrate buffer is recommended. Positive Control: Human Breast invasive ductal carcinoma. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | Highly purified (>98%) E.coli derived recombinant Human IL-2 (Cat.-No PA075) |
| Specificity | Detects Human Interleukin 2. Other species not tested. |
| Buffer | PBS without preservatives State: Azide Free State: Lyophilized (Sterile filtered) purified Ig fraction |
| Reconstitution Method | Restore in sterile water to a concentration of 1.0 mg/ml. |
| Purification | Affinity Chromatography on Protein A |
| Conjugation | Unconjugated |
| Storage | Prior to reconstitution store at 2-8°C. |
| Stability | Shelf life: One year from despatch. |
| Gene Name | interleukin 2 |
| Database Link | |
| Background | Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells. |
| Synonyms | IL-2, TCGF |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.