Mouse Anti-Human CD158e1-AF488
Référence 9850-30
Conditionnement : 100tests
Marque : Southern Biotech
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| Clone | DX9 |
|---|---|
| Isotype | Mouse (BALB/c) IgG1κ |
| Isotype Control | Mouse IgG1-AF488 (15H6) |
| Specificity | Human/Baboon CD158e1 |
| Alternative Names | NKB1, p70, KIR, 3DL1, KIR3DL1, KIR3D, NKAT3 |
| Description | CD158e1 is a 70 kDa transmembrane glycoprotein and a killer cell immunoglobulin (Ig)-like receptor (KIR) that is expressed on a subset of natural killer cells and T cells. The monoclonal antibody DX9 is expressed on a subset of CD3-, CD56+ NK cells isolated from peripheral blood at varying levels (<0.1% to 61%) among individuals but not on thymocytes, B lymphocytes, granulocytes, or monocytes. Interactions with CD158e1 and certain MHC HLA-B antigens on a target cell inhibits cytotoxicity, possibly preventing target cell lysis and death. KIR and MHC class I interactions may regulate NK and T cell response to antigen stimulation. |
| Immunogen | Human NK clone VL186-1 (CD3-, CD16+, CD56+) |
| Conjugate | AF488 (Alexa Fluor® 488) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | Lot specific |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications | Flow Cytometry – Quality tested 1,2,4,6-9 Immunohistochemistry-Frozen Sections – Reported in literature 2 Immunohistochemistry-Paraffin Sections – Reported in literature 3 Immunocytochemistry – Reported in literature 4 Immunoprecipitation – Reported in literature 1,4,9 Blocking – Reported in literature 1,5,7-9 |
| RRID Number | AB_2797125 |
| Gene ID | 3811 (Human) |
| Gene ID Symbol | KIR3DL1 (Human) |
| Gene ID Aliases | KIR; NKB1; NKAT3; NKB1B; NKAT-3; CD158E1; KIR3DL1/S1 |
| UniProt ID | P43629 (Human |
| UniProt Name | KI3L1_HUMAN (Human) |
Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup
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- 1. Litwin V, Gumperz J, Parham P, Phillips JH, Lanier LL. NKB1: a natural killer cell receptor involved in the recognition of polymorphic HLA-B molecules. J Exp Med. 1994;180:537-43. (Immunogen, Block, FC, IP)
- 2. Schleinitz N, Cognet C, Guia S, Laugier-Anfossi F, Baratin M, Pouget J, et al. Expression of the CD85j (leukocyte Ig-like receptor 1, Ig-like transcript 2) receptor for class I major histocompatibility complex molecules in idiopathic inflammatory myopathies. Arthritis Rheum. 2008;58:3216-23. (FC, IHC-FS)
- 3. Hisashi Y, Yamada K, Kuwaki K, Tseng Y, Dor FJ, Houser SL, et al. Rejection of cardiac xenografts transplanted from α 1,3-galactosyltransferase gene-knockout (GalT-KO) pigs to baboons. Am J Transplant. 2008;8:2516-26. (IHC-PS, Baboon Reactivity)
- 4. Purdy AK, Alvarez Arias DA, Oshinsky J, James AM, Serebriiskii I, Campbell KS. The AP-2 clathrin adaptor mediates endocytosis of an inhibitory killer cell Ig-like receptor in human NK cells. J Immunol. 2014;193:4675-83. (FC, IP, ICC)
- 5. Söderström K, Corliss B, Lanier LL, Phillips JH. CD94/NKG2 is the predominant inhibitory receptor involved in recognition of HLA-G by decidual and peripheral blood NK cells. J Immunol. 1997;159:1072-5. (Block)
- 6. D'Andrea A, Chang C, Franz-Bacon K, McClanahan T, Phillips JH, Lanier LL. Molecular cloning of NKB1. A natural killer cell receptor for HLA-B allotypes. J Immunol. 1995;155:2306-10. (FC)
- 7. Valiante NM, Uhrberg M, Shilling HG, Lienert-Weidenbach K, Arnett KL, D'Andrea A, et al. Functionally and structurally distinct NK cell receptor repertoires in the peripheral blood of two human donors. Immunity. 1997;7:739-51. (FC, Block)
- 8. Bakker AB, Phillips JH, Figdor CG, Lanier LL . Killer cell inhibitory receptors for MHC class I molecules regulate lysis of melanoma cells mediated by NK cells, γδ T cells, and antigen-specific CTL. J Immunol. 1998;160:5239-45. (FC, Block)
- 9. Yusa S, Catina TL, Campbell KS. SHP-1- and phosphotyrosine-independent inhibitory signaling by a killer cell Ig-like receptor cytoplasmic domain in human NK cells. J Immunol. 2002;168:5047-57. (FC, IP, Block)