Mouse GM-CSF ELISA Kit
Référence EA100240
Conditionnement : 1x96wells
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Mouse GM-CSF ELISA Kit
| Product Data | |
| ACCN | NM_009969 |
|---|---|
| Assay Length | 3.5 hours incubations; 1 hour washing and analyzing samples |
| Assay Platform | 96-well plate |
| Assay Type | Sandwich ELISA kit of Quantitative Detection for Mouse GM-CSF |
| Assay Type | Sandwich |
| Background | Granulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375. The standard product used in this kit is recombinant mouse GM-CSF, consisting of 125 amino acids with the molecular mass of 14.8KDa. |
| Components |
|
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Curve Range | 15.6pg/ml-1000pg/ml |
| figure_legend | Representative standard curve for Mouse GM-CSF PicoKine ELISA Kit |
| Gene Symbol | Csf2 |
| Interference | No significant interference observed with available related molecules. |
| Locus ID | 12981 |
| Reactivity | Mouse |
| Reactivity | Mouse |
| Reactivity | Mouse |
| Sample Type | Cell culture supernates, serum and plasma(heparin, EDTA). |
| Sample Volume | 100 ul |
| Sensitivity | <1pg/ml |
| Signal | Colorimetric |
| Species | Mouse |
| Specificity | This kit is used for quantitative detection of mouse GM-CSF |
| Standard Curve | /assets/images/elisa/kits/131/ek0365-1-e.jpg |
| Target Symbol | Csf2 |
| New | No |
| Description | Mouse GM-CSF ELISA Kit |