OSGIN1 (Oxidative Stress Induced Growth Inhibitor 1, BDGI, OKL38, Bone Marrow Stromal Cell-Derived Growth Inhibitor, Pregnancy Induced Growth Inhibitor, Ovary, kidney and liver protein 38)

The deduced 317aa protein has a calculated molecular mass of about 34.5kD. OKL38 does not have a transmembrane domain or signal peptide. Minor transcripts of 3.6 and 4.0 kb were also observed. In situ hybridization detected Okl38 in undifferentiated mammary epithelial cells of nonpregnant rats, and expression was upregulated in secretory epithelial of 18-day pregnant rats. Northern blot analysis detected reduced OKL38 expression in human breast cancer cell lines compared with normal mammary tissue. Expression was also reduced in chemically- induced rat mammary tumors. Overexpression of OKL38 in a human breast cancer cell line reduced the ability of these cells to form tumors in nude mice.||Applications: |Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.||Recommended Dilution:|ELISA: 1:100-1:5000 |Western Blot: 1:50-400 |Immunohistochemistry (frozen): 1:50-500 |Immunohistochemistry (paraffin): 1:10-100 |Immunocytochemistry: 1:50-500 |Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.