p53 antibody
Référence GTX102965-100ul
Conditionnement : 100ul
Marque : Genetex
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IP, ChIP assay |
Reactivity | Human, Mouse, Zebrafish |
Package | 100 μl, 25 μl |
APPLICATION
Application Note
Application | Recommended Dilution |
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WB | 1:500-1:3000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
IP | 1:100-1:500 |
ChIP assay | Assay dependent |
Calculated MW
Positive Control
Predict Reactivity
PROPERTIES
Form
Buffer
Preservative
Storage
Concentration
Antigen Species
Immunogen
Purification
Conjugation
RRID
Note
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Cellular Localization
Background
Database
DATA IMAGES
![]() | GTX102965 IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using p53(GTX102965) antibody at 1:500 dilution. |
![]() | GTX102965 WB ImageNon-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX102965) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | GTX102965 ICC/IF Image p53 antibody detects p53 protein at nucleus by immunofluorescent analysis. |
![]() | GTX102965 IHC-P Imagep53 antibody detects p53 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.p53 stained by p53 antibody (GTX102965) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
![]() | GTX102965 WB Image Sample (30 μg of whole cell lysate) |
![]() | GTX102965 WB ImageVarious whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX102965) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. |
![]() | GTX102965 WB ImageWhole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX102965) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | GTX102965 WB ImageVarious whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX102965) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | GTX102965 ICC/IF Imagep53 antibody detects p53 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 stained by p53 antibody (GTX102965) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200. |
![]() | GTX102965 ChIP assay Image p53 antibody immunoprecipitates p53 protein-DNA complex in ChIP experiments. ChIP Sample: HCT116 whole cell lysate/extract treated with CPT 500nM for 6hr |
![]() | GTX102965 IP ImageImmunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-p53 antibody (GTX102965). (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by GTX102965 diluted at 1:10000. EasyBlot anti-Rabbit IgG Kit (GTX225856-01) was used in Western blot. |
![]() | GTX102965 WB ImageThe data was published in the journal Cancer Cell Int in 2020.PMID: 31920463 |
![]() | GTX102965 WB ImageThe data was published in the journal Front Pharmacol in 2017.PMID: 29326587 |
Application Reference
REVIEW
p53 antibody Cat. No. GTX102965
Rating | | ( Average 4.7 based on 3 users reviews) |
Western Blot(WB) | | ( Average 4.5 based on 2 users reviews) |
IHC (Paraffin sections)(IHC-P) | | ( Average 5 based on 1 users reviews) |
SDS | |
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PBS.pdf | |
Glycerol.pdf | |
Thimerosal.pdf |