K1013-1ml | Phosphatase Inhibitor Cocktail 2 (100X in ddH2O) Clinisciences

Product Citation

1. Xiaojing Zhang, et al. "The effects of restraint stress on ceramide metabolism disorders in the rat liver: the role of CerS6 in hepatocyte injury." Lipids Health Dis. 2024 Mar 2;23(1):68. PMID: 38431645
2. Sung-Suk Suh, et al. "CREB5 promotes the proliferation and self-renewal ability of glioma stem cells." Cell Death Discov. 2024 Feb 28;10(1):103. PMID: 38418476
3. Aakarsha Rao, Zijian Ni, et al. "Targeted inhibition of BET proteins in HPV-16 associated head and neck squamous cell carcinoma reveals heterogeneous transcription response." bioRxiv. 2023 Oct 4:2023.10.02.560587. PMID: 37873389
4. Payal Deepak, Praveen Kumar, et al. "The triglyceride catabolism regulated by a serine/threonine protein phosphatase, Smek1, is required for development and plant infection inMagnaporthe oryzae." Int J Pharm. 2023 Jul 25:642:123160. PMID: 37379892
5. Tian Y, Wan N, et al. "Chemoproteomic mapping of the glycolytic targetome in cancer cells." Nature Chemical Biology, 2023: 1-12. PMID: 37322158
6. Haiping Hao, Yang Tian, et al. "Chemoproteomic Mapping of Glycolytic Targetome in Cancer Cells." Research Square. 05 Oct, 2022.
7. Rachel Shvartsur, Galila Agam, et al. "Low-Dose Aspirin Augments the Anti-Inflammatory Effects of Low-Dose Lithium in Lipopolysaccharide-Treated Rats." Pharmaceutics. 2022 Apr 20;14(5):901. PMID: 35631487
8. Thuy T P Nguyen, Do-Young Kim, et al. "SREBP-1c impairs ULK1 sulfhydration-mediated autophagic flux to promote hepatic steatosis in high-fat-diet-fed mice." Mol Cell. 2021 Sep 16;81(18):3820-3832.e7. PMID: 34233158

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Description

Protein phosphorylation is an important covalent post-translational modification that can alter the structural conformation of a protein, which then regulates the function, location and specific binding of the target protein. Many cellular processes are regulated by the reversible phosphorylation of proteins and 30% of the proteins are likely to be phosphorylated at some point during their existence.

Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading and modifying proteins in the extracts. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.

Phosphatase Inhibitor Cocktail 2 inhibits tyrosine protein phosphatases, acid and alkaline phosphatases. This phosphatase inhibitor cocktail has been optimized and tested on cell extracts from various animal tissues.

This phosphatase inhibitor cocktail contains individual components, including Sodium orthovanadate, Sodium molybdate, Sodium tartrate, Imidazole and Sodium Fluoride. This phosphatase inhibitor cocktail is supplied as a ready-to-use solution in ddH2O.

Quality Control

DataSheet

Protocol

Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.

Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Components and Storage

Catalog No.	Product Name	Summary	Targets	CAS Number	Smiles
A8524	Sodium Orthovanadate	PTP inhibitor	Membrane Transporter/Ion Channel\|ATPase	13721-39-6	[O-][V](=O)([O-])[O-].[Na+].[Na+].[Na+]
B7843	Sodium molybdate	Acid and phosphoprotein phosphatases inhibitor		7631-95-0	[O-][Mo](=O)([O-])=O.[Na+].[Na+]
B7844	Sodium tartrate	Acid phosphatases inhibitor		868-18-8	C(C(C(=O)[O-])O)(C(=O)[O-])O.[Na+].[Na+]
B7845	Imidazole	Alkaline phosphatases inhibitor		288-32-4	C1=CN=CN1
B7846	sodium fluoride	Acid phosphatases inhibitor		7681-49-4	[F-].[Na+]
Stored at -20°C, and stable for at least 12 months. When kept at 2-8°C, the product remains stable for 2 months.

Storage

Stored at -20°C, and stable for at least 12 months. When kept at 2-8°C, the product remains stable for 2 months.

Phosphatase Inhibitor Cocktail 2 (100X in ddH2O)

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