PKH67 Green Fluorescent Cell Linker Kit is a kit that uses the green fluorescent probe PKH67 for labeling cell membranes. PKH67 can bind to lipid regions of cell membranes and show fluoresce green. PKH67 is less toxic to cells, has a low fluorescence background, and does not stain neighboring cells, so it is widely used for cell tracing in vitro and in vivo. PKH67-labeled cells have good morphology and stable fluorescence expression, which can effectively observe the situation of cells in vitro; PKH67-labeled cells can also be used for in vivo observation for up to several weeks. Meanwhile, the fluorescence of PKH67 is evenly distributed to daughter cells with cell division, and the fluorescence signal is related to the cell cycle state. Based on this property, PKH67 can also be used to detect cell proliferation. PKH67 can also be used in combination with PKH26 for experiments such as labeling cells, detecting proliferation, and cell tracing.

Figure 1: Diagram of the kit for labeling Hela cells