PRNP ELISA Kit (Bovine)
Référence OKEH08763
Conditionnement : 96T
Marque : Aviva Systems Biology
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| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Bos taurus, Bovine | ||||||||||||||||||||||
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| Application | FA, FC, ICC, IP, WB | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology PRNP ELISA Kit (Bovine) (OKEH08763) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRNP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRNP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRNP captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: <=5.5% (n = 20) Mean Inter-assay CV%: <=8.9% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Function: Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as a agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains. | ||||||||||||||||||||||
| :: | Subcellular Location: Cell membrane Lipid-anchor GPI-anchor Golgi apparatus Targeted to lipid rafts via association with the heparan sulfate chains of GPC1. Colocates, in the presence of Cu(2+), to vesicles in para- and perinuclear regions, where both proteins undergo internalization. Heparin displaces PRNP from lipid rafts and promotes endocytosis. | ||||||||||||||||||||||
| :: | Unigene: Bt.4737 SMR: P10279 String: 9913.ENSBTAP00000043233 | ||||||||||||||||||||||
| :: | Pfam: PF00377 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sensitivity | 0.075 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Bovine Major prion protein | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | PRNP |
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| Gene Full Name | prion protein |
| Alias Symbols | alternative prion protein;AltPrP;major prion protein;major scrapie-associated fibril protein 1;prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia);prion protein precursor PrP;prion protein PrP;prion protein variant a;prion protein variant b;prn;PrP. |
| NCBI Gene Id | 281427 |
| Protein Name | P10279 |
| Description of Target | Major prion protein |
| Uniprot ID | https://www.uniprot.org/uniprot/P10279 |
| Protein Accession # | NP_001258555.1 |
| Nucleotide Accession # | NM_001271626.2 |