K1008-1ml | Protease Inhibitor Cocktail (EDTA-Free, 200X in DMSO)

Product Citation

1. Quanxin Jiang, Ning Wang, et al. "Targeting hepatic ceruloplasmin mitigates nonalcoholic steatohepatitis by modulating bile acid metabolism." J Mol Cell Biol. 2023 Sep 28:mjad060. PMID: 37771074
2. Md Shahjalal Hossain Khan, Marleigh Hefner, et al. "E4orf1 improves adipose tissue-specific metabolic risk factors and indicators of cognition function in a mouse model of Alzheimer's disease." Nutrition & Diabetes, 2023 Aug 12, 13(1): 13. PMID: 37573386
3. Xiaoxian Wu, Chengzhi Yu, et al. "The structural mechanism for transcription activation by Caulobacter crescentus GcrA." Nucleic Acids Res. 2023 Feb 28;51(4):1960-1970. PMID: 36715319
4. Junwan Lu, Yujie Li, et al. "Ciclopirox targets cellular bioenergetics and activates ER stress to induce apoptosis in non-small cell lung cancer cells." Cell Commun Signal. 2022 Mar 24;20(1):37. PMID: 35331268
5. Shi Du, Jiaxian Qian, et al. "Tumor cell-derived exosomes deliver TIE2 protein to macrophages to promote angiogenesis in cervical cancer." Cancer Lett. 2022 Mar 31;529:168-179. PMID: 35007697
6. Kathryn M Spitler, Shwetha K Shetty, et al. "Chronic high-fat feeding and prolonged fasting in liver-specific ANGPTL4 knockout mice." Am J Physiol Endocrinol Metab. 2021 Oct 1;321(4):E464-E478. PMID: 34396783
7. Kathryn M Spitler, Shwetha K Shetty, et al. "Regulation of plasma triglyceride partitioning by adipose-derived ANGPTL4 in mice." Sci Rep. 2021 Apr 12;11(1):7873. PMID: 33846453
8. Maria C Bewley, Lovely Gautam, et al. "Molecular architecture and domain arrangement of the placental malaria protein VAR2CSA suggests a model for carbohydrate binding." J Biol Chem. 2020 Oct 29;jbc.RA120.014676. PMID: 33122198
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10. Monteiro-Brás T, Wesolowski J, et al. "Depletion of SNAP-23 and Syntaxin 4 alters lipid droplet homeostasis during Chlamydia infection." Microb Cell. 2019 Dec 3;7(2):46-58. PMID: 32025513
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Quality Control

DataSheet

Protocol

It is suggested to dilute the cocktail appropriately for specific cell lines. The test should begin with at least 200 fold dilution because concentrations of DMSO greater than 0.5% may be deleterious for cells. Since various cell lines will differ in their sensitivity, further dilutions may be needed for this cocktail. This cocktail remains effective for up to 48 hours in the medium. After 48 hours, the medium should be replaced with fresh medium containing the cocktail.

Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Components and Storage

Catalog No.	Product Name	Summary	Targets	CAS Number	Smiles
A2574	Aprotinin	Inhibitor of bovine pancreatic trypsin	Proteases\|Serine Protease	9087-70-1	CC(O)=O.CC.CC.CCccC.[R].[P].[2H].1.[E].[P].[P].2024.[3H].0.[KH].[R].[R].2024.F.2024.[].1.F.[V].2024.0.0.[R].[KH].[R].N.N.F.[KH].S.[].[2H].[R].0.0.[].[KH].[].[3H].C.[].F.[P].[].I.I.N.[Q].[3H].Dec
A2575	Bestatin	Aminopeptidase inhibitor	Proteases\|Aminopeptidase	58970-76-6	CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O
A2576	E-64	Cysteine protease inhibitor,irriversible	Proteases\|Cathepsin	66701-25-5	CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O
A2570	Leupeptin, Microbial	Inhibitor of serine and cysteine proteases	Proteases\|Serine Protease	103476-89-7	O=C(C(C(00)(00)C(C(00)(00)00)(00)C(00)(00)00)(00)N(00)C(C(00)(00)00)=O)N(00)C(C(N(00)C(C(00)=O)(00)C(00)(00)C(00)(00)C(00)(00)/N=C(N(00)00)/N(00)00)=O)(00)C(00)(00)C(C(00)(00)00)(00)C(00)(00)00
A2571	Pepstatin A	Aspartic proteinases inhibitor	Proteases\|Other Proteases	26305-03-3	3-hydroxy-4-[2-[[3-hydroxy-6-methyl-4-[[3-methyl-2-[[3-methyl-2-(3-methylbutanoylamino)butanoyl]amino]butanoyl]amino]heptanoyl]amino]propanoylamino]-6-methylheptanoic acid
Stored at -20°C, and stable for at least 12 months.

Description

Secreted protein production is halted and degradation is increased when proteins are extracted from media in vitro. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading proteins in the extracts. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.

Protease inhibitor cocktail is used in tissue culture media to increase protein stability. The cocktail functions to inhibit proteases that would degrade either non-phosphorylated or phosphorylated protein substrates. This cocktail should be used as a supplement to tissue culture media to prevent the degradation of secreted proteins.

This protease inhibitor cocktail contains individual components, including Aprotinin, Bestatin, E-64, Leupeptin and Pepstatin A with a broad specificity for cysteine, serine, aspartic and aminopeptidases. This protease inhibitor cocktail has been optimized and tested for tissue culture media. This protease inhibitor cocktail is supplied as a ready-to-use solution in DMSO.

Storage

Stored at -20°C, and stable for at least 12 months.

Protease Inhibitor Cocktail (EDTA-Free, 200X in DMSO)

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