Proteinase K from Tritirachium album min. 30 mAnson-U/mg

Référence 33752.02

Conditionnement : 100MG

Marque : SERVA Electrophoresis

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EC 3.4.21.14Mr ca. 28 000CAS [39450-01-6]

Serin protease with very broad range of action: cleaves peptide bonds at the carboxylic side of aliphatic, aromatic, and hydrophobic amino acids. Suitable for the isolation of DNA and RNA (1, 3).

Unit definition: 1 mAnson unit is defined as the amount of enzyme that liberates folin-positive amino acids and peptides, corresponding to 1 µmol tyrosine per minute at 37 °C and pH 7.4 using urea-denatured hemoglobin as substrate (4).
Inhibitors for proteinase: AEBSF (cat. no. 12745), (PEFABLOC® SC (cat. no. 31682) and PMSF (cat. no. 32395)
Extraneous activities: DNases and RNases not detectable


References:
  1. Ebeling, W. et al.(1974) Eur. J. Biochem. 47, 91-7
  2. Lin, Y. et al.(1969) J. Biol. Chem. 244, 789-93
  3. Sambrook, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (B.16, 1.61)
  4. Anson, M.L. (1938) J. Gen. Physiol. 33, 79-89
DANGER
Hazard Statements H315 - H319 - H334 - H335
Precautions P280 - P284
Reaction P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311

EG-Index: 647-014-00-9EINECS: 254-457-8WGK: 1HS: 35079090
Storage Temperature: +2 °C to +8 °C

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Référence
Description
Cond.
Prix HT
PB0451-250mg
 250mg 
PB0451-1g
 1g 
PB0451-50mg
 50mg